FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2889446
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134290.4647603727496551No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86620.2997806499931129No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA73220.25340497797847755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71630.24790219301554692No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT65510.2267216622148329No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA63350.21924618075575736No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA58280.20169956455320504No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA53320.1845336441656982No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA48800.16889050703837344No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG45630.1579195458229709No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA44680.15463171832939604No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG41200.1425878870897743No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40180.13905779862298862No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA40030.1385386679661084No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38970.13487014465748798No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGT35950.12441831409896568No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT35860.12410683570483753No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT35580.12313779181199441TruSeq Adapter, Index 1 (95% over 22bp)
TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA34930.12088822563218No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA34330.11881170300465903No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA32450.1123052654384266No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT32320.11185535220246373No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT29070.10060752130339173No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)