FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4061460
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATACTATGCATTGGGTGCGCCAGGCCCCCGGACAAAGCCTTGAGTG194080.4778577161907294No Hit
GTGTATTACTGTGCGAGACTTGCTCGCCTGGAATTTCATGACTACTGGGG167240.4117731062228853No Hit
CTACCTATACTATGCATTGGGTGCGCCAGGCCCCCGGACAAAGCCTTGAG150520.3706056442757038No Hit
GTAATACACAGCCGTGTCTTCAGATCTCAGGCTGCTCAGCTCCATGTAGG132780.32692677017624205No Hit
ATACTATGCATTGGGTGCGCCAGGCCCCCGGACAAAGCCTTGAGTGGGTG129280.3183091794576335No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTGGGTGTTCGG124070.3054812801307904No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG123830.30489035962437155No Hit
GTATAGGTAGTGAAGGTGTATCCAGAAGCCTTGCAGGAAACCTTCACTGA104900.2582815046805828No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAAGACACGGCTGTGTATTA103250.2542189261989531No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA85310.21004761834414226No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA81590.20088835049464968No Hit
GTATTACTGTGCGAGACTTGCTCGCCTGGAATTTCATGACTACTGGGGCC79990.1969488804518572No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC77980.19199992121059917No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT77920.19185219108399443No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG77900.19180294770845951No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA75830.18670625834059673No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT73310.18050159302319854No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG70570.17375525057491642No Hit
ATGCATTGGGTGCGCCAGGCCCCCGGACAAAGCCTTGAGTGGGTGGGATG70500.17358289876054422No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT64970.1599671054251427No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC64400.15856366922239784No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC63070.1552889847493266No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA61560.15157110989644118No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA60530.14903507605639352No Hit
CTAATAATCAGCGGCCCTCAGGAGTCCCTGACCGATTCTCTGGCTCCAAG58720.1445785505704845No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC57400.1413284877851807No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT55750.13726590930355095No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA55410.13642877191945751No Hit
GCTGTGTATTACTGTGCGAGACTTGCTCGCCTGGAATTTCATGACTACTG54220.1334987910751306No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT53700.13221846331122306No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG52900.1302487282898268No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCATTCAGG52880.1301994849142919No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT52800.13000251141215227No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC51900.12778655951308152No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT50720.12488120035652203No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC49610.12214819301433474No Hit
ACTCCTTACCATGGACTGGACCTGGAGGATCCTCTTTTTGGTGGCAGCAG48940.12049853993391539No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA48430.11924283385777527No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT47930.11801174946940263No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA47290.11643596145228562No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA47280.11641133976451817No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC46760.11513101200061061No Hit
CTCCCAGGTCCAGCTTGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGG46540.11458933486972664No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT46200.11375219748563324No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC45820.11281657335047003No Hit
CAGTAATACACAGCCGTGTCTTCAGATCTCAGGCTGCTCAGCTCCATGTA45500.11202867934191152No Hit
ACTATAAACTGGTACCAGCAGCTCCCGGGAGCGGCCCCCAAACTCCTCAT45440.11188094921530682No Hit
TTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTGGGTGTTCGGCG45290.111511623898795No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC45190.11126540702112049No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG45160.11119154195781812No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG44510.10959113225293368No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT44290.1090494551220497No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44230.10890172499544498No Hit
CATCTATACTAATAATCAGCGGCCCTCAGGAGTCCCTGACCGATTCTCTG44160.10872937318107281No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC43960.10823693942572374No Hit
CCATTAGCAGGGACACATCCGCGAGCACAGCCTACATGGAGCTGAGCAGC43340.10671039478414167No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA43340.10671039478414167No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG42490.10461755132390815No Hit
ACATGGAGCTGAGCAGCCTGAGATCTGAAGACACGGCTGTGTATTACTGT42060.10355881874990766No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC41600.10242622111260483No Hit
GACCTGGAGGATCCTCTTTTTGGTGGCAGCAGCCACAGGTGCCCACTCCC41560.10232773436153501No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC41000.10094891984655764No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)