FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3750852
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTACACTGGTATCAACAGAAAGCAGATCAGTCGCCAAAGATCTTCGT151480.4038549108309259No Hit
GTAATAGATGGCGGTGTCGGAGTCCCTCAGACTACTCCACTGCAAATATG140660.3750081314858597No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT132270.35263988021921416No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC129990.3465612612814368No Hit
ATCTATTACTGTGCGCGAGTCCCCGCCAGCAGCTGGTCCGTGGGCTACTT124390.3316313200307557No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC111230.2965459580916549No Hit
ACATATTACTGTCAACAGACTTATATTTTACCATTCACTTTCGGCCCTGG96630.257621468402379No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA84080.22416240363522744No Hit
GACTTATATTTTACCATTCACTTTCGGCCCTGGGACCAAAGTGGGTATCA74860.19958132179035587No Hit
GGTATCAACAGAAAGCAGATCAGTCGCCAAAGATCTTCGTCAAGTATGCT71930.19176976324312447No Hit
GTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGT69210.18451807749279364No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT68160.1817187135082909No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC67020.17867940403940225No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG64760.17265410632037734No Hit
ATATAAGTCTGTTGACAGTAATATGTTGCAGCATCTTCAGCTTCCAGGCT64620.17228085778911031No Hit
ATATTTTACCATTCACTTTCGGCCCTGGGACCAAAGTGGGTATCAAACGA63270.1686816755233211No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT62970.1678818572420346No Hit
ATATTACTGTCAACAGACTTATATTTTACCATTCACTTTCGGCCCTGGGA61740.16460260228876No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT60870.16228312927302918No Hit
GTAATATGTTGCAGCATCTTCAGCTTCCAGGCTAGTGATGGTGAGGGTGA58450.15583126180398482No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC58340.15553799510084643No Hit
GTCTGGAGCAGAGGTGAGAAAGCCCGGGGAGTCCCTGAAGATCTCCTGTA57970.1545515525539264No Hit
CAGTAATAGATGGCGGTGTCGGAGTCCCTCAGACTACTCCACTGCAAATA53350.14223435102211443No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGG53050.14143453274082796No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG52280.13938166581885927No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT52140.13900841728759225No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG49000.1306369859434603No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC47900.1277043189120765No Hit
CATTTACCCAGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG47330.1261846641776322No Hit
TCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTC47320.12615800356825596No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA47300.12610468234950353No Hit
GTTCAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCGTGCACCAGG46920.12509157919320732No Hit
CTATTACTGTGCGCGAGTCCCCGCCAGCAGCTGGTCCGTGGGCTACTTTG46900.1250382579744549No Hit
TATTTGGACTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATC45770.12202560911494242No Hit
CCTCCTGGCTGTTCTCCAAGGAATCTGTGCCGAGGTACAGTTGGTGCAGT45670.12175900302118026No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG45290.12074589986488403No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT45170.12042597255236943No Hit
GTCTTCCTGTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGAC43980.11725336003659968No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG43150.11504052945837372No Hit
ACAGAAAGCAGATCAGTCGCCAAAGATCTTCGTCAAGTATGCTTCCCAGT42470.11322760802079102No Hit
TCCCAGTCCTTCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGG42360.11293434131765263No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC41770.11136136536445587No Hit
CCTGTAAGTGTTCTGGATACAGATTTAGCACCTACTGGATCAACTGGGTG41500.11064152891129803No Hit
GATTTAGCACCTACTGGATCAACTGGGTGCGCCAGATGCCCGGGAAAGGC41280.11005499550502126No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT40840.10888192869246774No Hit
GTACGTGCTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA40730.10858866198932934No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA40350.10757555883303313No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAA39760.10600258287983637No Hit
CACCATCACTAGCCTGGAAGCTGAAGATGCTGCAACATATTACTGTCAAC39600.10557601312981692No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA38690.10314989767658121No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG38460.10253670366092824No Hit
ATCATGGGGTCAACCGCCATCCTCGCCCTCCTCCTGGCTGTTCTCCAAGG38290.10208347330153256No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA38220.10189684903589905No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA38140.10168356416088932No Hit
GTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAAAACCATCTCCAAAGC37830.1008570852702266No Hit
GTCCAAATATGGTCCCCCGTGCCCATCATGCCCAGCACCTGAATTCCTGG37650.10037719430145473No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC37600.10024389125457363No Hit
GTGTAAACTACTACCAATGCTCTGACTGGCCCGGCAGGTGATGGTGACAT37570.10016390942644497No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC37560.10013724881706876No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)