FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3071625
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT97960.3189191388922802No Hit
GTCTCTAATTACTCCATCAGAAATTATTACTTCTGGGGCTGGATCCGGCA90460.2945020957961991No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT80900.2633785048630611No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79500.2588206568184593No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC78630.2559882798193139No Hit
CTATAACCCGTCCCTCAAGAATCGACTCACCATGTCAATAGACACGTCCA78100.2542628087738575No Hit
GTTTATTACTGTCAACAATTTTTTACTACTCCATTCACTTTCGGCCCTGG66820.21753957595735157No Hit
CAATAATAGAAGGCCGTGTCTGCGGCGGTCACAGAGGTCAGGTTCAGGGA62480.203410247019086No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA58050.18898791356366743No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA54230.17655149961339683No Hit
CTATTATTGTGCGAGAGGACGTCGACGGGGAGACTACGACTCTTGGGGCC48280.15718064542383917No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT45880.14936719163309323No Hit
TTATTACTGTCAACAATTTTTTACTACTCCATTCACTTTCGGCCCTGGGA44550.14503723599072152No Hit
CTCCTAAGCTGCTCATTCACTGGGCATCTATGCGGGAATCCGGGGTCCCT43490.14158629389980873No Hit
ATTAGAGACAGTGCAGGTGAGGGACAGGGTCTCCGAAGGCCTCACCAGTC42710.13904692141781633No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42440.13816790786635738No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41660.13562853538436495No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC40470.13175436454645342No Hit
ATAGAAGGCCGTGTCTGCGGCGGTCACAGAGGTCAGGTTCAGGGAGAACT39410.12830342245554063No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA38020.12377813046840028No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG37920.12345256989378585No Hit
TATTTGGACTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATC37910.12342001383632442No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36520.11889472184918407No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36400.11850404915964675No Hit
CACCTGCACTGTCTCTAATTACTCCATCAGAAATTATTACTTCTGGGGCT36060.11739714320595776No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA33400.10873723192121433No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT31890.10382126724453669No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGA31430.10232368860131039No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA31320.10196557196923452No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC31250.10173767956700444No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC30760.10014243275139381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)