FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2231994
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA83700.37500100806722597No Hit
GTATATTACTGTGCGAAAGCCCTCTTAGTGGCTACGATTACCTCGCGGAA56840.25466018277826913No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC46820.20976758898097395No Hit
GATTATTACTGCAGCTCATATGGAGGCAGGAAAAATTTGGTGTTCGGCGG38570.17280512402811118No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37830.16948970292930896No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG37270.16698073561129645No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT36120.16182839201180646No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT36010.1613355591457683No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA34740.15564557969241852No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTC33580.15044843310510692No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT32880.14731222395759128No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA32370.14502727157868703No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA31180.13969571602791048No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAAATTTTTCCTG31110.1393820951131589No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTG30840.1381724144419743No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30490.13660430986821648No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA30270.13561864413614017No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA28900.12948063480457384No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT28600.1281365451699243No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA28380.12715087943784795No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG27960.12526915394933857No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC27780.12446270016854885No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC27580.1235666404121158No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG26980.12087846114281668No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAA26330.11796626693440931No Hit
ATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT26120.11702540419015464No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25350.11357557412788745No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC25260.11317234723749257No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTAGTTATAACTATGTCTCCT25230.11303793827402762No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA24930.11169384863937805No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT24900.11155943967591311No Hit
GCCGTATATTACTGTGCGAAAGCCCTCTTAGTGGCTACGATTACCTCGCG24780.11102180382205329No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT24400.10931929028483052No Hit
GTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAA23740.1063622930886015No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC23680.10609347516167159No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC23220.1040325377218756No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23180.10385332577058899No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC22960.10286766003851265No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT22960.10286766003851265No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)