FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4551249
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACA232380.5105851163054361No Hit
GTATATTACTGTGCGAGAGATATGCAGCAGCTAGTCCCTTACAACCGCTA226660.498017137713186No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT133620.29358973767420765No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC130650.2870640564820778No Hit
CATTAGTAGTGGTCGTAATTTCATTTACTACGCAGACTCTGTGAAGGGCC103260.22688277437687984No Hit
CAGTTATACTACCCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGG102020.22415824754918925No Hit
ATATACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACAGTG95980.21088716525947052No Hit
GTTATACTACCCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGGTC95190.20915137800634506No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA91210.20040652576908008No Hit
CTCCAGAGACAGCGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGA88810.19513324803806603No Hit
CTACTAATGGATGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGAC86940.19102448580598425No Hit
GGCTACAGCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTG83560.18359795300147277No Hit
GTATAACTGTGTTGACAGGAGTAAGTTGCAAAATCTTCAGGTTGCAGATT81860.17986271460867118No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT80860.17766551555408197No Hit
GCTGTATATTACTGTGCGAGAGATATGCAGCAGCTAGTCCCTTACAACCG80400.17665480398897093No Hit
ATATTACTGTGCGAGAGATATGCAGCAGCTAGTCCCTTACAACCGCTACT77570.17043673066448353No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC76830.16881080336408752No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA73620.16175779439885624No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT68800.15116729495573633No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT68760.15107940699355277No Hit
GTCTCTGGAGATGGTGAATCGGCCCTTCACAGAGTCTGCGTAGTAAATGA68500.15050813523935958No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC68380.15024447135280888No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC67000.1472123366574758No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG66890.14697064476147098No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG66470.1460478211585435No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG65270.14341118229303648No Hit
CTCCTGATCTATGCTGCAGTCACTTTGCAAAGTGGGGTCCCATCAAGGTT64750.1422686387846501No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC63630.1398077758435102No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG63420.13934636404204648No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT63180.1388190362689451No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG60730.13343589858520155No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGCCCTGGTCAAGCCGGGGG59330.13035981990877668No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGGCAT56130.12332878293409127No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT56000.12304314705699469No Hit
CTTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATC54110.11889044084382111No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC53810.11823128112744435No Hit
CAGTAATATACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATA53640.1178577572881642No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG53430.11739634548670046No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT51770.11374899505608241No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCGTCAGCCTCC50540.11104644021893771No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT50430.1108047483229329No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA47890.10522386272427633No Hit
CCCTGAGACTCTCCTGTGCAGCCTCCGGATTCACTTGCACTGGCTACAGC47590.10456470300789959No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG47420.10419117916861942No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG47360.10405934722534409No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA47180.10366385139551802No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA46690.10258722385876931No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG45920.10089538058673565No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG45730.1004779127663637No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA45550.10008241693653763No Hit
TAGTATAACTGTGTTGACAGGAGTAAGTTGCAAAATCTTCAGGTTGCAGA45540.10006044494599174No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)