FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4551249
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACA228470.5019940680019924No Hit
GTATATTACTGTGCGAGAGATATGCAGCAGCTAGTCCCTTACAACCGCTA203340.44677845576016606No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT138490.304290097070057No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG132210.29049168700723693No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC113260.24885476492277175No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT105500.2318045002591596No Hit
CAGTTATACTACCCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGG101290.22255429223933915No Hit
GTTATACTACCCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGGTC97240.21365563606825289No Hit
CATTAGTAGTGGTCGTAATTTCATTTACTACGCAGACTCTGTGAAGGGCC96460.21194182080567334No Hit
ATATACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACAGTG90640.19915412230796428No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA87850.1930239369456604No Hit
CTCCAGAGACAGCGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGA84360.18535571224514413No Hit
GTATAACTGTGTTGACAGGAGTAAGTTGCAAAATCTTCAGGTTGCAGATT81520.17911566693011083No Hit
GGCTACAGCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTG78390.17223843388924664No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC77510.1703048987212082No Hit
GCTGTATATTACTGTGCGAGAGATATGCAGCAGCTAGTCCCTTACAACCG76310.16766825985570116No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA75940.16685529620550316No Hit
CTACTAATGGATGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGAC75410.16569078070657087No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT75130.16507556497128592No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC74510.16371330155744063No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA71880.15793466804387102No Hit
ATATTACTGTGCGAGAGATATGCAGCAGCTAGTCCCTTACAACCGCTACT69650.15303491415213713No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT67820.14901403988223894No Hit
CTCCTGATCTATGCTGCAGTCACTTTGCAAAGTGGGGTCCCATCAAGGTT66620.14637740101673188No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT64390.141477647124998No Hit
GTCTCTGGAGATGGTGAATCGGCCCTTCACAGAGTCTGCGTAGTAAATGA64170.14099426333298837No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG63520.13956608394750541No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC63040.13851142840130257No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT58480.12849220071237588No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT58200.12787698497709088No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG58130.12772318104326966No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG57570.1264927495726997No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC54970.12078003203076781No Hit
CTTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATC53340.11719859757178742No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC53030.11651746586486479No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGCCCTGGTCAAGCCGGGGG52630.1156385862430291No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGGCAT51160.112408703632783No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG50990.11203517979350283No Hit
CAGTAATATACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATA50330.11058502841747397No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC49070.10781655760869159No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG49070.10781655760869159No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA48830.10728922983559018No Hit
CTCCTGTGCAGCCTCCGGATTCACTTGCACTGGCTACAGCATGAACTGGG47660.10471850694172083No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA47350.1040373752347982No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG46960.1031804676035084No Hit
CCCTGAGACTCTCCTGTGCAGCCTCCGGATTCACTTGCACTGGCTACAGC46900.10304863566023305No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG46890.10302666366968716No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC46870.10298271968859538No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC46780.10278497177368234No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT46590.10236750395331039No Hit
ATACTACCCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGGTCAAA46550.10227961599112684No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC46230.10157651229365829No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT46100.10129087641656169No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG45660.10032410883254245No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)