FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4484296
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG69980.15605571086297604No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTCATGTGGTATT68170.15201940282265042No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA65480.1460206908732162No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG61060.1361640712388299No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG56220.12537084973873266No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG55760.12434504769533503No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC55230.12316314534098552No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA47150.10514470944826121No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA46520.10373980664969484No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)