FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4324942
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC121140.2800962417530686No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT119990.2774372465572949No Hit
ATAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA70080.16203685506071527No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTACCAC66500.15375928740778488No Hit
GAGTTACAGTATCCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCA65740.15200203840883877No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT59070.13657986627335117No Hit
GATCTATGCTGCATCCCGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG58420.13507695594530517No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC56520.13068383344793988No Hit
GTGTATTATTGTACAAGAGGGCGCGCGGAGTGGCTGGTACAAGGTGACCA56370.13033700798762157No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG56160.12985145234317594No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG55070.12733118733152954No Hit
GTATTATTGTACAAGAGGGCGCGCGGAGTGGCTGGTACAAGGTGACCATA52370.12108832904579993No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA50610.1170189103113984No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT49430.11429055002356102No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT48850.11294949157699688No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT47770.11045234826270503No Hit
CAATAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATA46240.10691472856745823No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC45930.10619795594946707No Hit
GTACAAGAGGGCGCGCGGAGTGGCTGGTACAAGGTGACCATAGTGCTTTT45160.1044175852531664No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC45050.1041632465822663No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)