FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3324892
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATTACTGTGCAAGAGAAATGCCTCCCGACTACGGTGACTACGCAGA174540.5249493818145071No Hit
GTAATACACAGCCGTGTCTTCGGCTCTCAGACTGTTCATTTGCAGAGACA151870.4567667160316786No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC110970.333755201672716No Hit
CATTAATAGTGATGGGAGTATCACAAGCTACGCGGACTCCGTGAGGGGCC91960.2765804122359463No Hit
ACTCAGGACCCCGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCAC85180.25618877244734567No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATTGAGGGTTACCACTG83490.25110590058263543No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC78850.23715056007834237No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT74390.2237365905418883No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA73390.22072897405389408No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA73090.2198266891074958No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA72190.21711983426830106No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC71630.21543556903502428No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA68230.205209672975844No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT68030.2046081496782452No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC67110.20184114250929053No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGTTGACTCAGGACCCCGCT65990.19847261204273703No Hit
CTATTATTGTAACTCCCGGGACAGCAGTGGTAACCCTCAATTCGGCGGAG65410.1967281944797004No Hit
ATCACAAGCTACGCGGACTCCGTGAGGGGCCGATTCACCATCTCCAGAGA64220.1931491308589873No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTG63960.19236715057210882No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC62640.18839709680795647No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGTTGACTCAGGA60780.18280293014028726No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG60620.1823217115022082No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTG60400.18166003587484947No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG59850.18000584680645265No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT59840.17997577064157272No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA58500.1759455645476605No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGTC57710.17356954752214507No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCCGGGGGAGGCT56920.17119353049662966No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT56810.17086269268295032No Hit
TTGCATAGGTTCTGTGGTTTCTTCTGAGTTGACTCAGGACCCCGCTGTGT55270.16623096329143924No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATTGAGGGTTACCACTGC55000.1654189068396808No Hit
GTCTGAGAGCCGAAGACACGGCTGTGTATTACTGTGCAAGAGAAATGCCT54730.1646068503879224No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG54060.16259174734096626No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA53970.16232106185704678No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATTGAGG53280.1602458064803308No Hit
GTGTGAGGTGCAGCTGGTGGAGTCCGGGGGAGGCTTAGTTCAGCCTGGGG52950.1592532930392927No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG52900.159102912214893No Hit
GTATTACTGTGCAAGAGAAATGCCTCCCGACTACGGTGACTACGCAGATG51940.15621560038641857No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG51250.15414034500970256No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC50850.15293729841450487No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT50780.1527267652603453No Hit
CTCCAGAGACAACGCCAGGAACACGCTGTCTCTGCAAATGAACAGTCTGA50170.15089211920266885No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT50080.15062143371874936No Hit
CCCCTGAACTTGTCATCTATGGTCAAAACAACCGGCCCTCAGGGATCCCA49770.14968907260747116No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA49620.14923793013427203No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC48550.14601978049211822No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC48280.1452077240403598No Hit
CCCTGAGACTCTCCTGTGCAGGCTCTGGATTCACCTTCAATACCTACTGG48110.14469642923740078No Hit
CAATAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA47420.1426211738606848No Hit
GCTGTGTATTACTGTGCAAGAGAAATGCCTCCCGACTACGGTGACTACGC47390.14253094536604496No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT46750.1406060708137287No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG45870.13795936830429378No Hit
CTATTAATGCGTGAGACCCACACCAGCCCCTTCCCTGGAGTTTGGCGGAC45860.13792929213941385No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC45300.1362450269061371No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA45090.1356134274436583No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC44860.13492167565141966No Hit
GAGTATCACAAGCTACGCGGACTCCGTGAGGGGCCGATTCACCATCTCCA44730.13453068550798042No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA44710.13447053317822052No Hit
TCTCAGAACTTATTATGCAAGCTGGTTCCAGGAGAAGCCAGGGCAGGCCC44400.13353817206694232No Hit
ATACACAGCCGTGTCTTCGGCTCTCAGACTGTTCATTTGCAGAGACAGCG44060.1325155824610243No Hit
CTCCTGTGCAGGCTCTGGATTCACCTTCAATACCTACTGGATGCACTGGG43400.13053055557894813No Hit
GTCAGGATCACATGCCAAGGAGACAGTCTCAGAACTTATTATGCAAGCTG42900.12902674733495104No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG42600.12812446238855277No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG41760.12559806453863764No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT41600.12511684590055858No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA40480.12174831543400508No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG40140.12072572582808705No Hit
GTCTCAGAACTTATTATGCAAGCTGGTTCCAGGAGAAGCCAGGGCAGGCC40010.1203347356846478No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA39480.11874069894601087No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA39380.11843993729721146No Hit
CCTTCAATACCTACTGGATGCACTGGGTCCGCCAAACTCCAGGGAAGGGG38320.11525186381993761No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC37400.11248485665098294No Hit
GTCTCTGCAAATGAACAGTCTGAGAGCCGAAGACACGGCTGTGTATTACT37100.11158257170458469No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG36830.11077051525282626No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA36790.11065021059330649No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG36710.11040960127426695No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC36680.11031937277962713No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT36470.10968777331714834No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC36450.10962762098738846No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT36300.10917647851418934No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT36300.10917647851418934No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG35900.10797343191899167No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG35880.10791327958923178No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA35840.107792974929712No Hit
GTTCAGGGGCCTGCCCTGGCTTCTCCTGGAACCAGCTTGCATAATAAGTT35780.10761251794043235No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA35400.10646962367499456No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT35260.10604855736667537No Hit
GACTATTATTGTAACTCCCGGGACAGCAGTGGTAACCCTCAATTCGGCGG35260.10604855736667537No Hit
ACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGG35030.10535680557443669No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC34530.10385299733043961No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA34470.10367254034115995No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC34300.10316124553820094No Hit
CTTCTGAGTTGACTCAGGACCCCGCTGTGTCTGTGGCCTTGGGACAGACA34240.1029807885489213No Hit
CAGTAATACACAGCCGTGTCTTCGGCTCTCAGACTGTTCATTTGCAGAGA33830.10174766578884367No Hit
CTCCGTGAGGGGCCGATTCACCATCTCCAGAGACAACGCCAGGAACACGC33700.10135667564540442No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC33440.10057469535852592No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)