FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3366445
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT102400.3041784434321666No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC102090.3032575907225575No Hit
CTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCA88730.263571809431017No Hit
ATCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGT78920.23443127691080648No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG72900.21654891138872012No Hit
ATATTACTGTCAACAGTTTGATAATCTCCCGCTCACCTTCGGCCAAGGGA68390.2031519897102136No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA58770.17457585078621513No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAA58340.17329853896320896No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA54420.16165420792557134No Hit
ATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACC52530.15603997688956747No Hit
GTGTATTACTGTGGGAGAGATCGCTCCGCACGCAGTGGCTGGTACGGCGG51740.15369328772637011No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT51580.15321800890850734No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAATAGACACGTCC50810.1509307295975428No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC50810.1509307295975428No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT50780.15084161481919353No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49280.14638587590173016No Hit
CTATAATAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG49020.1456135478227032No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA48990.14552443304435392No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG45860.13622679116991365No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45400.13486036456855824No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT44750.13292954437099078No Hit
ACATATTACTGTCAACAGTTTGATAATCTCCCGCTCACCTTCGGCCAAGG44470.13209780643973093No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA43480.1291570187542051No Hit
GTATTACTGTGGGAGAGATCGCTCCGCACGCAGTGGCTGGTACGGCGGCC43390.1288896744191573No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG42910.127463837965569No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT42690.12681032959100774No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG42470.12615682121644642No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42210.12538449313741945No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC42130.12514685372848805No Hit
ATAATAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA42000.12476068968897457No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG41410.12300809904810565No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC39860.11840383550006015No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG39810.11825531086947805No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT36120.10729419313251813No Hit
CAATAGACACGTCCAAGAACCAGTTCTCCCTGAAACTGAGCTCTGTGACC36020.10699714387135391No Hit
ATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATC35760.10622481579232693No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT35570.10566042219611489No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA35510.10548219263941634No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC35220.1046207497820401No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGT35070.10417517589029378No Hit
AGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAA34730.10316520840233541No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAATAGACAC34210.10162055224428143No Hit
CAGTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGTTTCAGGGA33960.10087792909137087No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)