FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3243563
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT128470.3960767834631237No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC124330.3833130418616811No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTACTAC65980.20341827798627618No Hit
GATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG65880.2031099750490433No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT63820.19675893454204527No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG61620.1899762699229212No Hit
AGTCAGAACATTACTACCTATTTAAATTGGTATCAGCAGAAATCAGGGAA59840.1844884776401753No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC57170.17625678921605653No Hit
GAGTTACAATAGCCAAGCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGA56950.17557852275414412No Hit
CATTACTACCTATTTAAATTGGTATCAGCAGAAATCAGGGAAAGCCCCTA55530.17120062104543676No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG53260.16420214437024963No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT52030.1604100182422848No Hit
GTAATACGTGGCTGTGTCCTCAGGGTCCATGTTGGTCATTGTAAGGACCA49670.15313406892358802No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC48590.14980439720147257No Hit
GTATTACTGTGCACGGACTCTAGAGATTTTAGATGATTACTTTTGGGGGA47560.14662887694797355No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA46210.14246678729532924No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT46170.14234346612043608No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG45040.13885964292970415No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT44480.13713314648119984No Hit
CTAGTGGAATGTGTGTGAGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTG44330.1366706920753505No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC44320.13663986178162718No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG44210.13630072855067096No Hit
CAGTAATACGTGGCTGTGTCCTCAGGGTCCATGTTGGTCATTGTAAGGAC43780.13497502592056945No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA43730.13482087445195298No Hit
ATTTAAATTGGTATCAGCAGAAATCAGGGAAAGCCCCTAAGCTCCTGATC43440.13392679593397755No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA43010.132601093303876No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA42550.1311828997926046No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCC41830.12896311864452764No Hit
GAACATTACTACCTATTTAAATTGGTATCAGCAGAAATCAGGGAAAGCCC41760.1287473065884646No Hit
ACTTACTACTGTCAACAGAGTTACAATAGCCAAGCGCTCACTTTCGGCGG41750.1287164762947413No Hit
TTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT41500.12794571895165902No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC40950.126250052796878No Hit
TCTCTGAAGACCAGGCTCACCATCTCCAAGGACACCTCCAAAAACCAGGT39870.12292038107476254No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG39180.12079309080785543No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG38660.11918991553424428No Hit
CTCTAGAGATTTTAGATGATTACTTTTGGGGGAGTTATCGTACATACGGT38540.1188199520095648No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT37900.11684681321127416No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG37620.11598356498702198No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA37260.1148736744129835No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC36570.11274638414607639No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT36300.11191396621554753No Hit
AGTGGAATGTGTGTGAGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTGGA36170.11151317239714474No Hit
TCTCTAGAGTCCGTGCACAGTAATACGTGGCTGTGTCCTCAGGGTCCATG35960.11086573622895562No Hit
CCCTCACACTGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGA35930.11077324534778574No Hit
GCTACTGACTGTCCCGTCCTGGGTCTTATCCCAGGTCACCTTGAGGGAGT35430.10923173066162117No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG35100.10821433096875256No Hit
AGCCCACACAGACCCTCACACTGACCTGCACCTTCTCTGGGTTCTCACTC34800.10728942215705384No Hit
TCCATACCGTATGTACGATAACTCCCCCAAAAGTAATCATCTAAAATCTC34260.1056245862959961No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34040.10494631983408369No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG33770.10411390190355482No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC33770.10411390190355482No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC33660.10377476867259863No Hit
ACGTATTACTGTGCACGGACTCTAGAGATTTTAGATGATTACTTTTGGGG33450.1031273325044095No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC33340.10278819927345331No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC32820.10118502399984214No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)