FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3243563
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT129410.39897483107311316No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC106380.3279726646283732No Hit
GATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG67320.20754953734519727No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT65110.20073604243234988No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTACTAC64320.19830044922820983No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT59910.18470428969623837No Hit
AGTCAGAACATTACTACCTATTTAAATTGGTATCAGCAGAAATCAGGGAA59530.18353273853475327No Hit
GAGTTACAATAGCCAAGCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGA58550.18051136974987073No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57100.1760409771599935No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG56140.17308126896255754No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA55350.17064567575841752No Hit
GTAATACGTGGCTGTGTCCTCAGGGTCCATGTTGGTCATTGTAAGGACCA54940.16938163371576256No Hit
CATTACTACCTATTTAAATTGGTATCAGCAGAAATCAGGGAAAGCCCCTA54830.16904250048480637No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG51670.1593001276682463No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49930.15393565656039362No Hit
CAGTAATACGTGGCTGTGTCCTCAGGGTCCATGTTGGTCATTGTAAGGAC49850.1536890142106073No Hit
CCCTCACACTGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGA48060.14817039163413814No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA47170.1454264954927652No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT46980.14484071991202266No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG46440.14317588405096496No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT46340.14286758111373202No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT46300.14274425993883885No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC45570.1404936484970386No Hit
ATTTAAATTGGTATCAGCAGAAATCAGGGAAAGCCCCTAAGCTCCTGATC45260.13953790939161656No Hit
GTATTACTGTGCACGGACTCTAGAGATTTTAGATGATTACTTTTGGGGGA44910.13845884911130138No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA44750.13796556441172872No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42320.13047380303696893No Hit
TCCATACCGTATGTACGATAACTCCCCCAAAAGTAATCATCTAAAATCTC41690.12853149453240156No Hit
TTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT41650.1284081733575084No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC41260.12720579190230002No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG41140.12683582837762056No Hit
TCTCTAGAGTCCGTGCACAGTAATACGTGGCTGTGTCCTCAGGGTCCATG41060.12658918602783423No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA40810.1258184286847519No Hit
ACTTACTACTGTCAACAGAGTTACAATAGCCAAGCGCTCACTTTCGGCGG40680.12541763486634913No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40580.12510933192911622No Hit
GAACATTACTACCTATTTAAATTGGTATCAGCAGAAATCAGGGAAAGCCC40340.12436940487975724No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT39690.1223654357877433No Hit
CTAGTGGAATGTGTGTGAGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTG39200.12085475139530202No Hit
TCTCTGAAGACCAGGCTCACCATCTCCAAGGACACCTCCAAAAACCAGGT38800.11962153964637037No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT38800.11962153964637037No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA38660.11918991553424428No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG37140.114503710888304No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC36640.11296219620213943No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCC36180.11154400269086805No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC35960.11086573622895562No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC35130.10830682184992245No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG35070.1081218400875827No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA34930.10769021597545661No Hit
CTCTAGAGATTTTAGATGATTACTTTTGGGGGAGTTATCGTACATACGGT34770.10719693127588395No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA34460.10624119217046192No Hit
AGTGGAATGTGTGTGAGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTGGA34340.10587122864578244No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT34130.10522379247759331No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG33950.10466884719057407No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)