FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3561973
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCTCAGATTTCAGGCTGCTCAGCTCCATGTAGA110710.3108108904812024No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG109170.30648744389696386No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG99200.2784973384132895No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC91710.25746966638994734No Hit
GTCTATTACTGTGCGAGAGATTGTGGTGGTGGTGACTTCATTATTTTGGG83020.23307307495031546No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG72800.20438111125491407No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT70270.1972783061522364No Hit
GTCTACATGGAGCTGAGCAGCCTGAAATCTGAGGACACGGCCGTCTATTA67000.18809800074284674No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA60800.17069191709201614No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT56650.15904107077734728No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA56170.15769350301083138No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCACATGAATGCCA56030.1573004624122642No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT54500.15300509015649472No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT52420.14716562983492576No Hit
GGTTAACAGTGATGGCAGCCACAACAAGGGGGACGGGATCCCTGATCGCT52300.14682873789329676No Hit
ACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG49430.13877140562267035No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCATTCATGTGGTGTTCGGCGG49100.13784495278319064No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG48690.13669390531595832No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC47660.13380224948364292No Hit
ATTCATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGGAGTGC46990.13192126947621444No Hit
CTATTACTGTCAGACCTGGGGCACTGGCATTCATGTGGTGTTCGGCGGAG46270.1298999178264406No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG46090.1293945799139971No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA46000.12914191095777536No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT45940.12897346498696088No Hit
CTACAGAAGCCTCTGAGAGGAAAGTTCTTCACCATGGACTGGACCTGGAG45620.12807508647595028No Hit
GTACTTGATGAAGGTTAACAGTGATGGCAGCCACAACAAGGGGGACGGGA43130.12108457868714895No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG43040.12083190973092722No Hit
GATATGAATACCTCCCAAAATAATGAAGTCACCACCACCACAATCTCTCG42560.1194843419644113No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC42520.11937204465053497No Hit
GTTCTGGGGCTGAGCGCCACCTCACCATCTCCAGCCTCCAGTCTGAGGAT42460.11920359867972048No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA42380.11897900405196783No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG42040.11802447688401906No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAAC41780.11729454434382294No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41010.11513282105170364No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG40630.11406599656987855No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTGGC40220.1129149491026462No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA39760.11162352999306843No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT39580.11111819208062497No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT39570.1110901177521559No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT39440.11072515148205783No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA39380.11055670551124334No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCAC39010.10951795535788732No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA37700.10584021832843764No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC36290.10188173801429713No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA36150.10148869741573No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC35830.10059031890471938No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA35790.10047802159084306No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCC35770.1004218729339049No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)