FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3561973
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCTCAGATTTCAGGCTGCTCAGCTCCATGTAGA146520.4113450607289836No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG109910.3085649442036759No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG91910.258031152959329No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC89780.2520513209954146No Hit
GTCTATTACTGTGCGAGAGATTGTGGTGGTGGTGACTTCATTATTTTGGG73230.20558830737908454No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70600.19820475899171608No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG68360.19191610941464182No Hit
GTCTACATGGAGCTGAGCAGCCTGAAATCTGAGGACACGGCCGTCTATTA62000.17406083650830592No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT59080.16586313259533408No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT57790.16224154422282258No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCACATGAATGCCA57680.16193272660966268No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG55700.15637400957278452No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA54590.15325775911271647No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC54050.15174174537538604No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA53450.15005728566724116No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT50310.14124194652794952No Hit
GATATGAATACCTCCCAAAATAATGAAGTCACCACCACCACAATCTCTCG46950.1318089721623381No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG45500.1277381945343213No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCATTCATGTGGTGTTCGGCGG44500.12493076168741313No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC44430.12473424138812955No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA44350.1245096467603769No Hit
ACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG44090.12377971422018078No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC43570.12231984913978855No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA43160.1211688016725562No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG42580.11954049062134946No Hit
ATTCATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGGAGTGC42570.11951241629288038No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42110.11822099718330262No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC41720.11712609837300846No Hit
CTATTACTGTCAGACCTGGGGCACTGGCATTCATGTGGTGTTCGGCGGAG41330.11603119956271427No Hit
GTGCATATAGTAGCTGGTGAAGATGTATCCAGATGTCTTGCAGGAAACCT41280.11589082792036885No Hit
GGTTAACAGTGATGGCAGCCACAACAAGGGGGACGGGATCCCTGATCGCT41080.11532934135098723No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT40540.11381332761365683No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCAC40480.11364488164284234No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT40340.11325184104427517No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA40260.11302724641652252No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG39770.11165160432153752No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTGGC39420.11066900282511967No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA39390.11058477983971243No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT39100.10977062431410906No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG38890.10918106341625834No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA37670.10575599534303039No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT36770.10322930578081306No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCG36650.10289241383918409No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA36430.10227477861286427No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT36420.1022467042843952No Hit
TATGAATACCTCCCAAAATAATGAAGTCACCACCACCACAATCTCTCGCA36390.10216248129898794No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA35820.10056224457625029No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGC35780.10044994726237397No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)