FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4542907
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTACACCGACCCCGTTATAAAAACTCCCTGAACCATAGTAATACCCT269710.5936947421551884No Hit
GTGTACACTACTATTACTACATGGACGTCTGGGGCAAAGGGACCACGGTC243440.5358683327657818No Hit
GTGTACTACTGTGCGAGAGGGTATTACTATGGTTCAGGGAGTTTTTATAA224880.4950134352299089No Hit
GTAGTACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA222750.4903248074415787No Hit
AGTACATACTATGCAGACTCCGCGAAGGGCCGATTCAGCATCTCCAGGGA196110.431683941581899No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC130690.2876792327027606No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT123930.27279889286749653No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG123200.27119199226398427No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT115440.2541104187252788No Hit
ATACTATGCAGACTCCGCGAAGGGCCGATTCAGCATCTCCAGGGACAATT103750.22837799673204842No Hit
CTATGCAGACTCCGCGAAGGGCCGATTCAGCATCTCCAGGGACAATTTCA101790.22406357867330323No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA99140.21823030935918344No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT95920.21114233683410202No Hit
TAGTAATACCCTCTCGCACAGTAGTACACAGCCGTGTCCTCAGCTCTCAG95360.20990964596017486No Hit
GTATGTACTTCCACCACTATAAATGACTGAGACCCACTCCAGCCCCTTCC94950.20900714014176386No Hit
CTACTATTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCT90120.19837518135414176No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC85590.18840359267755205No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC85500.18820548164424233No Hit
CATTTATAGTGGTGGAAGTACATACTATGCAGACTCCGCGAAGGGCCGAT82440.18146970651171157No Hit
GTATTACTATGGTTCAGGGAGTTTTTATAACGGGGTCGGTGTACACTACT82000.18050116368219732No Hit
GTAATAGTAGTGTACACCGACCCCGTTATAAAAACTCCCTGAACCATAGT79290.17453582034587103No Hit
GTGTACACCGACCCCGTTATAAAAACTCCCTGAACCATAGTAATACCCTC78770.1733911788200815No Hit
TGTGTGAGTTGTGTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCA78090.17189433990174133No Hit
CCTATGAACATCCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA77730.1711018957685024No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGG73230.16119634410301598No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA71460.15730016044792464No Hit
GCTGTGTACTACTGTGCGAGAGGGTATTACTATGGTTCAGGGAGTTTTTA70580.1553630747888962No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG70250.1546366676667605No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT70050.15419642092607222No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC68130.1499700522154647No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCGTCAGTAGCAACTAC68070.1498379781932582No Hit
CTCCTGTGCAGCCTCTGGATTCACCGTCAGTAGCAACTACATGGGCTGGG67440.1484512009600901No Hit
GTACTACTGTGCGAGAGGGTATTACTATGGTTCAGGGAGTTTTTATAACG66610.1466241769862337No Hit
GTACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACAATA66230.14578770817892597No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG66190.1456996588307883No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCCGAGAGTGCTG65590.14437891860872343No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGT64750.14252988229783264No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGG64040.14096700636838924No Hit
GTAGTGTACACCGACCCCGTTATAAAAACTCCCTGAACCATAGTAATACC63560.13991041419073735No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG62280.13709283505033232No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAGGCCCCCAA61460.13528782341351034No Hit
GAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT61440.1352437987394415No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTTCAAC60630.13346079943965394No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC59330.13059919562518008No Hit
ACTATTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCC59260.1304451092659392No Hit
GTACACTACTATTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCAC58250.12822186322546336No Hit
CTCCTGGGAGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGATAC57650.12690112300339848No Hit
GTAATACCCTCTCGCACAGTAGTACACAGCCGTGTCCTCAGCTCTCAGGC56740.1248980003332668No Hit
GTGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT55310.12175023613734554No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAATCTGGGGGAGGCT54900.12084773031893455No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC54850.12073766863376248No Hit
TGCCCACGGTGCCCAGAGCCCAAATCTTGTGACACACCTCCCCCATGCCC54800.1206276069485904No Hit
GACCTACACCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA54400.11974711346721384No Hit
GATTATTACTGCAGCTCATATACAACCACCAGCACTCTCGGGATATTCGG54010.11888863232287167No Hit
CAGTAGTACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATA54010.11888863232287167No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGTTATCT53980.11882259531176843No Hit
GTGCCCAGCACCTGAACTCCTGGGAGGACCGTCAGTCTTCCTCTTCCCCC53940.11873454596363078No Hit
GATCTGCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGG53850.11853643493032104No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA53700.11820624987480483No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGG53360.11745783041563473No Hit
TCCTGGAGCAGGGCGCCAGGGGGAAGACCGATGGGCCCTTGGTGGAAGCT53330.11739179340453151No Hit
GTTCAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG52320.11516854736405566No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCT51890.11422201687157583No Hit
GGGTATTACTATGGTTCAGGGAGTTTTTATAACGGGGTCGGTGTACACTA51570.11351762208647459No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAA51490.11334152339019926No Hit
ATGCAGACTCCGCGAAGGGCCGATTCAGCATCTCCAGGGACAATTTCAAG51300.11292328898654541No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT51120.11252706691992594No Hit
GTGTGAGGTGCAGCTGGTGGAATCTGGGGGAGGCTTGGTCCAGCCTGGGG51040.11235096822365062No Hit
GTCATTTATAGTGGTGGAAGTACATACTATGCAGACTCCGCGAAGGGCCG50080.11023778386834684No Hit
GGTGTACACTACTATTACTACATGGACGTCTGGGGCAAAGGGACCACGGT49980.1100176604980027No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGTTATCTGCCTTCCAG49690.1093793027240047No Hit
GTACATACTATGCAGACTCCGCGAAGGGCCGATTCAGCATCTCCAGGGAC48340.10640763722435877No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC48100.10587934113553282No Hit
GGGCTGGAGTGGGTCTCAGTCATTTATAGTGGTGGAAGTACATACTATGC47910.10546110673187895No Hit
CTACAACACCACGCCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCT47860.10535104504670686No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC47800.1052189710245004No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCC47650.10488878596898417No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA46960.10336993471360958No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC45820.10086052829168636No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA45770.1007504666065143No Hit
GTGTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCACCTTGGTGTT45680.10055235557320456No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)