FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4542907
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA207070.4558094629716171No Hit
GTGTACACTACTATTACTACATGGACGTCTGGGGCAAAGGGACCACGGTC204970.4511868721943901No Hit
GTGTACTACTGTGCGAGAGGGTATTACTATGGTTCAGGGAGTTTTTATAA201650.4438787762989645No Hit
AGTGTACACCGACCCCGTTATAAAAACTCCCTGAACCATAGTAATACCCT197820.4354480512147838No Hit
AGTACATACTATGCAGACTCCGCGAAGGGCCGATTCAGCATCTCCAGGGA197550.4348537181148546No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT141890.3123330501813046No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC141650.3118047540924787No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT106720.23491566083126947No Hit
CTATGCAGACTCCGCGAAGGGCCGATTCAGCATCTCCAGGGACAATTTCA105550.232340217398243No Hit
ATACTATGCAGACTCCGCGAAGGGCCGATTCAGCATCTCCAGGGACAATT102880.2264629234100544No Hit
TAGTAATACCCTCTCGCACAGTAGTACACAGCCGTGTCCTCAGCTCTCAG93510.2058373636088082No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC89070.19606388596552826No Hit
CATTTATAGTGGTGGAAGTACATACTATGCAGACTCCGCGAAGGGCCGAT85580.18838158034051763No Hit
CTACTATTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCT80740.17772760921586112No Hit
GTATGTACTTCCACCACTATAAATGACTGAGACCCACTCCAGCCCCTTCC80590.1773974241603449No Hit
TGTGTGAGTTGTGTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCA78350.17246666066463612No Hit
CTCCTGTGCAGCCTCTGGATTCACCGTCAGTAGCAACTACATGGGCTGGG75180.16548874982472678No Hit
GTATTACTATGGTTCAGGGAGTTTTTATAACGGGGTCGGTGTACACTACT74790.1646302686803846No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCGTCAGTAGCAACTAC74100.16311141742501No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAACTGCT73270.16128439345115364No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGG73170.1610642700808095No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG71810.15807059224412914No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA70080.15426245793717547No Hit
TCATAAGGGTATCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT69440.15285366836697295No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA69370.15269958200773204No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGG69100.15210524890780286No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT68080.1498599905302926No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC67730.1490895587340881No Hit
CCTATGAACATCCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA67000.14748265813057584No Hit
GTGTTGTAGTTGTTCTCCGGCTGCCCGCTGCTCTCCCACTCCACGGCGAT66280.14589776986409803No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA65630.1444669679568611No Hit
GTACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACAATA63850.14054877196473536No Hit
GTAATAGTAGTGTACACCGACCCCGTTATAAAAACTCCCTGAACCATAGT63670.1401525498981159No Hit
GCTGTGTACTACTGTGCGAGAGGGTATTACTATGGTTCAGGGAGTTTTTA63560.13991041419073735No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT63520.1398223648425997No Hit
GTACTACTGTGCGAGAGGGTATTACTATGGTTCAGGGAGTTTTTATAACG63190.13909595772046401No Hit
GAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT62900.138457599946466No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC62460.13748905711695175No Hit
GTGTACACCGACCCCGTTATAAAAACTCCCTGAACCATAGTAATACCCTC62340.13722490907253881No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCCGAGAGTGCTG62060.1366085636355752No Hit
GGGGAGGTGTGTCACAAGATTTGGGCTCTGGGCACCGTGGGCATGTGTGA61760.13594819352454277No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA61340.13502367536909737No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAATCTGGGGGAGGCT59070.1300268748622853No Hit
GTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGATGTTCCCCTGCT55990.12324707505568572No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAGGCCCCCAA55550.12227853222617148No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT55320.12177224847437994No Hit
GATCTGCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGG55060.12119992771148518No Hit
ACTATTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCC53920.11869052128956195No Hit
GTGTGAGGTGCAGCTGGTGGAATCTGGGGGAGGCTTGGTCCAGCCTGGGG53650.11809618818963276No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53620.11803015117852952No Hit
CAGTAGTACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATA53320.1173697810674971No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG53000.11666538628239582No Hit
GTAATACCCTCTCGCACAGTAGTACACAGCCGTGTCCTCAGCTCTCAGGC52900.11644526291205168No Hit
TCCTGGAGCAGGGCGCCAGGGGGAAGACCGATGGGCCCTTGGTGGAAGCT52580.11574086812695043No Hit
GATTATTACTGCAGCTCATATACAACCACCAGCACTCTCGGGATATTCGG52520.11560879410474395No Hit
GTACACTACTATTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCAC51920.11428805388267908No Hit
ATGCAGACTCCGCGAAGGGCCGATTCAGCATCTCCAGGGACAATTTCAAG51870.11417799219750702No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGAAGCGGTTGTGCAG51590.11356164676054341No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCT51540.11345158507537134No Hit
GTCATTTATAGTGGTGGAAGTACATACTATGCAGACTCCGCGAAGGGCCG50960.11217486952737532No Hit
GTAGTGTACACCGACCCCGTTATAAAAACTCCCTGAACCATAGTAATACC50930.11210883251627207No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC50790.11180065979779026No Hit
GTACATACTATGCAGACTCCGCGAAGGGCCGATTCAGCATCTCCAGGGAC50520.11120632669786108No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGT49820.10966546310545208No Hit
GTCCACGTACCACTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCA49350.10863088326483461No Hit
GTGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT48680.10715605668352886No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGTTATCT48600.10697995798725354No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC48180.10605543983180814No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGG46520.10240139188409536No Hit
CCGTGGGCATGTGTGAGTTGTGTCACCAAGTGGGGTTTTGAGCTCAACTC46360.10204919449154472No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)