FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3493309
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACATGCAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTATATTA115290.33003092483373214No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT109960.3147731849658877No Hit
GTAATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTGCATGTAGA95690.27392366378124583No Hit
GTATATTACTGTGCGAGAGATTCCCAGGGGTTTTTTGGAGTCAACTACTA92360.26439115463304275No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT92250.2640762669434625No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC91430.2617289223484095No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAGC58630.16783513854628948No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC54220.15521100480948008No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT51650.1478540833347408No Hit
ATTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG51580.14765370025955335No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTAAAACGTTCGGCCAAGGGA51240.14668041103721427No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT50980.14593613104366088No Hit
CATGTAGACTGTGCTCGTGGACGTGTCCCTGGTCATGGTGACTCTGTCCT49550.14184259107911726No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA48810.1397242557128499No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG48800.1396956295592517No Hit
TTCCAGGACAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTA47910.13714790188901124No Hit
CTACAGAAGCCTCTGAGAGGAAAGTTCTTCACCATGGACTGGACCTGGAG44830.12833104658076339No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT44710.1279875327375849No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA43080.12332146970107712No Hit
CTACTACGGTGTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT42990.12306383431869325No Hit
GGATACACCTTCACCAATTATTATATGCACTGGGTGCGACAGGCCCCTGG42440.12148939587079186No Hit
GCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC42370.12128901279560439No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC41610.11911342512214065No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41370.11842639743578366No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA41240.11805425743900697No Hit
GCCGTATATTACTGTGCGAGAGATTCCCAGGGGTTTTTTGGAGTCAACTA40570.11613630514792708No Hit
CCGTAGTAGTAGTAGTTGACTCCAAAAAACCCCTGGGAATCTCTCGCACA40110.11481950208240954No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37890.10846449598360752No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT37340.10689005753570613No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG36960.10580226369897425No Hit
ATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTGCATGTAGACTG35580.10185185450242162No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC35180.10070680835849333No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)