FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2948630
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT97480.33059420815768675No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC96440.32706714643749807No Hit
ATATTATTGTGTCACACAGGAGGAGCGATTTAGTGACTACCAATGGGTGT73580.24953961670335037No Hit
GCCGTATATTATTGTGTCACACAGGAGGAGCGATTTAGTGACTACCAATG72300.24519861766311812No Hit
ACAGTACCCCGTACACTTTTGGCCGGGGGACCAAGCTGGAGATCAAACGA70880.24038282185286047No Hit
GTATATTATTGTGTCACACAGGAGGAGCGATTTAGTGACTACCAATGGGT70570.23933148614780422No Hit
ATAATATACGGCTGTGTCTTCGGTTTTCAGGCTGCTCATTTCCAGATACA70000.2373983850127008No Hit
ATAGGAGACAGAGTCACCATCACATGCCGGGCAAGTCAGAGCACTAGCGG69560.235906166592621No Hit
ATATACGGCTGTGTCTTCGGTTTTCAGGCTGCTCATTTCCAGATACACCG67120.22763113717217828No Hit
CCCTTAGAGTCTCCTGTGCAGCCTCTGGATTCACCTTGAGTGACACCTGG60150.2039930408359136No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA59170.2006694634457358No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA55660.18876563014009895No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC53070.17998188989462904No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT52700.17872706985956188No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT51770.17557306274439316No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT48810.1655345024638561No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC48690.16512753380383433No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC47820.16217701101867646No Hit
GATTAGGAGGTTAGGGGCTTGCCCTGGTTTCTGCTGATACCAATTTAAAT46780.15864994929848777No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46740.15851429307848053No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG46260.15688641843839343No Hit
GAGTTACAGTACCCCGTACACTTTTGGCCGGGGGACCAAGCTGGAGATCA45390.15393589565323557No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG44730.15169756802311582No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG43730.14830616252293438No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGC43390.1471530846528727No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC41690.14138769530256423No Hit
CAATAATATACGGCTGTGTCTTCGGTTTTCAGGCTGCTCATTTCCAGATA41640.14121812502755518No Hit
CTCCTAATCTATGCTACATCCACTTTGCAAAGTGGGGGCTCATCAAGGTT40470.1372501805923429No Hit
AATCTATGCTACATCCACTTTGCAAAGTGGGGGCTCATCAAGGTTCAGTG39310.13331615021213242No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38710.13128130691202355No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACAT37790.12816121385185664No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA37730.12795772952184573No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT37630.1276185889718276No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG37630.1276185889718276No Hit
CTATAGGAGACAGAGTCACCATCACATGCCGGGCAAGTCAGAGCACTAGC35920.12181928556651733No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAG35450.12022532498143207No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT35380.11998792659641937No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT35160.11924181738637944No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA34820.11808873951631774No Hit
ATGCTACATCCACTTTGCAAAGTGGGGGCTCATCAAGGTTCAGTGGCAGT34630.11744437247128328No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA34450.11683391948125062No Hit
ATCACATGCCGGGCAAGTCAGAGCACTAGCGGCTATTTAAATTGGTATCA33640.11408688102610363No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG33170.11249292044101837No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG33100.11225552205600567No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC32800.11123810040595125No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGATT32570.1104580771409095No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG32500.1102206787558968No Hit
GTGGGGGCTCATCAAGGTTCAGTGGCAGTGGATCCGGGACAGATTTCACT31440.10662578892570448No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA31410.10652404676069903No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCCGGCCAAAAGTG31020.10520139861562829No Hit
CCTCCTAATCTATGCTACATCCACTTTGCAAAGTGGGGGCTCATCAAGGT30760.1043196331855811No Hit
TATTAAAACCAAGGCTGATGGTGGGACAACAGACTACGCTGCATCCGTGA30580.10370918019554844No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30280.10269175854549402No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT30170.10231870394047406No Hit
ATTTAAATTGGTATCAGCAGAAACCAGGGCAAGCCCCTAACCTCCTAATC30000.10174216500544321No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA29740.10086039957539603No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)