FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005107357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2796802
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATTACTGTGCGAGAGAGAACGGATGGAACTGGTTCGACCCCTGGGG106320.3801484695734628No Hit
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGAAGATACA100700.36005409035033586No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTACAGAGA88370.31596802347824404No Hit
GATTATTACTGCAGCTCATATGCAGGCAGCAACAATTATGTCTTCGGAAC81640.2919048255829336No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC72680.2598682352200835No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC71540.25579215117838155No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT64620.2310496059427875No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC59280.21195637016849958No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC57850.2068433875547858No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTC55510.19847668873234503No Hit
ATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTACAGAGACAATT55310.19776158626888854No Hit
GTAATAATCAGCCTCATCGTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG55120.19708223892860488No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA53740.1921480319307552No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT51070.18260141404361124No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA50980.18227961793505582No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT50850.18181480133380912No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC50390.18017006566785923No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA49280.1762012469956758No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC48860.17469953182241718No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA48110.1720178975844554No Hit
TCTCTGGGCTCCAGGCTGACGATGAGGCTGATTATTACTGCAGCTCATAT48000.17162459122955434No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA44550.1592890737349301No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT44140.15782311368484434No Hit
GTATTACTGTGCGAGAGAGAACGGATGGAACTGGTTCGACCCCTGGGGCC43940.15710801122138784No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG43860.15682197023600528No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATGACTATGTCTCCT42910.153425233534587No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT41820.1495279251087492No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT41660.14895584313798402No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC41500.14838376116721883No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG40920.14630996402319507No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG40810.14591665766829398No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATTGTTGCTGC38810.13876563303372924No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT38710.138408081802001No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA38460.13751420372268042No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG38340.1370851422446065No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT38050.13604824367259463No Hit
GGTTATGACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAA37950.1356906924408664No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC36910.13197215963089273No Hit
GCTGTGTATTACTGTGCGAGAGAGAACGGATGGAACTGGTTCGACCCCTG36760.13143583278330037No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36030.1288257087916842No Hit
GTTATTTATAGTGGTGGTAGCACATACTACGCAGACTCCGTGAAGGGCCG35560.1271452180025615No Hit
CAGTTATTTATAGTGGTGGTAGCACATACTACGCAGACTCCGTGAAGGGC34840.12457084913411819No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG34560.12356970568527911No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG34480.12328366469989652No Hit
ATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGAAGATACAGCG34470.12324790957672371No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATG33990.12153166366442815No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC32560.11641868105071436No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATTGTTGCTG32320.11556055809456658No Hit
CTCCTGTGCAGCCTCTGGATTCACCGTCAGTAGCAACTACATGAGCTGGG31520.11270014824074066No Hit
GCTCATATGCAGGCAGCAACAATTATGTCTTCGGAACTGGGACCAAGGTC31280.1118420252845929No Hit
GTATGTGCTACCACCACTATAAATAACTGAGACCCACTCCAGCCCCTTCC31240.11169900479190159No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT30780.11005426912595172No Hit
CTACGCAGACTCCGTGAAGGGCCGATTCACCATCTACAGAGACAATTCCA30460.10891010518442135No Hit
CAGTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGAAGATA30350.10851679882952028No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGT30190.10794471685875512No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA30000.10726536951847145No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA29520.10554912360617591No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT29490.10544185823665743No Hit
CTACAGAGACAATTCCAAGAACACGCTGTATCTTCAAATGAACAGCCTGA29470.1053703479903118No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC29290.10472675577320097No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG29240.10454798015733684No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCGTCAGTAGCAACTAC29210.10444071478781837No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTC29120.10411891867926296No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG29080.10397589818657166No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA28940.10347532646215213No Hit
CATATGCAGGCAGCAACAATTATGTCTTCGGAACTGGGACCAAGGTCACC28160.10068642685467186No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGG28150.10065067173149905No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC27970.10000707951438823No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)