FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2423926
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAACTGGATCCGGCATCC76470.3154799280176045No Hit
ATAATAGACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA72290.29823517714649705No Hit
GATTATTACTGCTGCTCATATGCAGGTTCTAGCGCTTTCGCGTTATTTGG64000.2640344630982959No Hit
GCCCAGGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC62250.2568147707479519No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59480.24538702914197874No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT58130.23981755218599907No Hit
GCCCTGGGCTGACCGACAACGGTCAGCTTGGTCCCTCCGCCAAATAACGC55140.22748219211312556No Hit
CTTATAACCTTGTCTCCTGGTACCGACAGCACCCAGGCAAAGCCCCCAAG54370.2243055274789742No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGGCCAGAGATTGTCAGGGAGG52320.2158481735828569No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA46780.19299269037091066No Hit
ATCTTTTACAGTGGGAGCACCAACTACAACCCCTCCCTGAAGAGTCGAGT45330.1870106595663399No Hit
GTCTATTATTGTGCGAGAGAGGAGGCTTACTACTTTGACTACTGGGGCCA44590.18395776108676584No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA41250.17017846254382352No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC40770.16819820407058633No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC40760.1681569486857272No Hit
GGGTATATCTTTTACAGTGGGAGCACCAACTACAACCCCTCCCTGAAGAG39350.16233993942059288No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA39070.16118478864453784No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT38970.16077223479594674No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA38620.15932829632587792No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAAGCAGCAGTGATATT38580.15916327478644152No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38280.15792561324066826No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT37120.1531399885970116No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC36400.1501696008871558No Hit
CTATTATTGTGCGAGAGAGGAGGCTTACTACTTTGACTACTGGGGCCAGG35260.1454664870132174No Hit
CTTTTACAGTGGGAGCACCAACTACAACCCCTCCCTGAAGAGTCGAGTCA34270.14138220391216563No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC34230.1412171823727292No Hit
CAATAATAGACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGA33980.14018579775125148No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT33720.13911315774491465No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT33520.13828805004773248No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG33100.1365553238836499No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA32950.13593649311076328No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC32860.1355651946470313No Hit
CTCCTGCACTGGAAGCAGCAGTGATATTGGGACTTATAACCTTGTCTCCT31850.13139840077626133No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAACTGGA31580.1302845053850654No Hit
TCCCTGAAGAGTCGAGTCACCATGTCAGCAGACACGTCCAAGAATCAGTT30710.12669528690232293No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA30180.12450875150479017No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC29850.12314732380443957No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29730.12265225918613026No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29340.12104329917662503No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG28290.11671148376641861No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA28140.11609265299353198No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG27480.1133697975928308No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA27310.11266845605022596No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT26130.10780032063685112No Hit
CTACAACCCCTCCCTGAAGAGTCGAGTCACCATGTCAGCAGACACGTCCA25940.10701646832452806No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT25630.10573755139389569No Hit
GTGATATTGGGACTTATAACCTTGTCTCCTGGTACCGACAGCACCCAGGC25540.10536625293016372No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT25270.10425235753896778No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC25250.10416984676924956No Hit
TATTGGGACTTATAACCTTGTCTCCTGGTACCGACAGCACCCAGGCAAAG25240.10412859138439046No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA24540.10124071444425284No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCCTGGGCTGA24470.10095192675023909No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)