FastQCFastQC Report
Mon 8 Aug 2022
EGAF00005107379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5492564
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTACTGTGCGAGAGATCCCGTGGCTGGCCAGTTTGACTACTGGGG243200.4427804573601691No Hit
GTAATATACGGCAGTGTCGTCAGCCTGTAGGCTGCCGATCTGCAGATATG210430.3831179755028799No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT152290.27726577241521444No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC151100.2750992068549406No Hit
GCTCCACACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAG143510.26128052399571494No Hit
GCCGTATATTACTGTGCGAGAGATCCCGTGGCTGGCCAGTTTGACTACTG135930.24748004756976888No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT111290.20261939596880438No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG108010.19664768585309156No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA105290.19169553600103703No Hit
ATATACGGCAGTGTCGTCAGCCTGTAGGCTGCCGATCTGCAGATATGCCG99470.18109939183230273No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG98900.18006162513536483No Hit
ATATTACTGTGCGAGAGATCCCGTGGCTGGCCAGTTTGACTACTGGGGCC96560.17580131974793556No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA96530.17574670044809673No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG95390.17367116705422095No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA90150.16413099601570413No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC89480.1629111649859701No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG88730.1615456824899992No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT84440.15373512261304556No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG81950.14920172072642213No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACGGGA81720.14878297276099103No Hit
CCTAAGAAGAAGCCCCTAGACCACAGCTCCACACCATGGACTGGACCTGG80970.14741749026502013No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT78880.14361234570958117No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT77930.14188273454801803No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT77440.14099061931731702No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT74290.13525559283423916No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG73740.1342542390038605No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG73520.13385369747170903No Hit
GAGTTACAGTACCCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCA71980.13104990674664874No Hit
CTCCCAGGTGCAGTTGGTGCAATCTGGGTCTGAGTTGAAGAAGCCTGGGG71680.1305037137482604No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC70470.1283007353214273No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC70340.12806405168879234No Hit
GTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGC68850.1253512931301301No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTG68790.12524205453045245No Hit
GACCTGGAGGATCCTCTTCTTGGTGGCAGCAGCAACAGGTGCCCACTCCC67240.12242005737211255No Hit
CAACCACACCCCTCCTAAGAAGAAGCCCCTAGACCACAGCTCCACACCAT65820.11983474384640762No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG64990.1183236098841998No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC64970.11828719701764058No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT64950.11825078415108134No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCA64340.11714019172102501No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT63910.11635731509000169No Hit
GTACGGGACCCAACAACCACACCCCTCCTAAGAAGAAGCCCCTAGACCAC63730.11602959929096865No Hit
GTATCAACGCAGAGTACGGGACCCAACAACCACACCCCTCCTAAGAAGAA63600.1157929156583337No Hit
CCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAGCAACAGGT63280.11521030979338612No Hit
ACCACACCCCTCCTAAGAAGAAGCCCCTAGACCACAGCTCCACACCATGG63200.11506465832714921No Hit
GATCAACACCAACACGGGAAACCCAACGTATGCCCAGGGCTTCACAGGAC62160.11317118926606955No Hit
ATCTGGGTCTGAGTTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA62020.11291629920015496No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG61860.11262499626768117No Hit
CCCCTAGACCACAGCTCCACACCATGGACTGGACCTGGAGGATCCTCTTC60950.11096821083923646No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC60640.11040381140756848No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG60320.10982120554262088No Hit
CTCCTAAGAAGAAGCCCCTAGACCACAGCTCCACACCATGGACTGGACCT60190.10958452190998594No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA59530.10838289731353154No Hit
GATCTATGCTGCATCCAGTTTGCAAAGGGGGGTCCCATCAAGGTTCAGTG59070.10754540138266937No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG58400.10632557035293534No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC57310.10434106912545763No Hit
GGTATGCTCTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGG57270.10426824339233917No Hit
GAGTTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGTCTTCTGG56600.10304841236260516No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG56420.10272069656357212No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC55600.10122776903464394No Hit
CCTTCACTAGGTATGCTCTGAATTGGGTGCGACAGGCCCCTGGACAAGGG55490.1010274982685682No Hit
GCTCTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGG55050.10022641520426527No Hit
GGATACACCTTCACTAGGTATGCTCTGAATTGGGTGCGACAGGCCCCTGG55000.1001353830378672No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)