FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3718246
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTGGCTCCATCAGCAGTAGTACCGACTACTGGGGCTGGATCCG171010.45992115637319314No Hit
ATAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA147180.39583179811126No Hit
ATATAATAGTAGGCTCCCTGAACCCCCCGGAGGCGTCTCGCACAATAATA126450.34007970424764794No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT115670.31108753966251834No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA107980.29040574507442485No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT107930.2902712730680003No Hit
CTATTATATGGATGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAG107650.28951822983202297No Hit
GTGTATTATTGTGCGAGACGCCTCCGGGGGGTTCAGGGAGCCTACTATTA98650.26531326867560673No Hit
GTATTACTGTCAGCAGTATGGTAGTTCTTTCGGCGGAGGGACCAAGGTGG95110.2557926506207497No Hit
ATTATATGGATGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGCC92700.2493110999110871No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC92380.2484504790699701No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC88900.2390912274228225No Hit
ACCTACTACAACGCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACAC86880.2336585583632713No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT85080.22881756613198803No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA84240.22655843642405585No Hit
GTATTATTGTGCGAGACGCCTCCGGGGGGTTCAGGGAGCCTACTATTATA80270.2158813591139478No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC78290.21055626765953622No Hit
CCATATAATAGTAGGCTCCCTGAACCCCCCGGAGGCGTCTCGCACAATAA77690.20894260358244182No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGAAGCCACCCTCTCCT76330.20528496500769447No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT76110.20469328817942653No Hit
TCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGAC74760.2010625440059641No Hit
CAATAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA73610.1979696878581998No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTACCGACTACTGGG72470.19490372611172044No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC71140.1913267707408278No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT70270.1889869578290409No Hit
ATAGTAGGCTCCCTGAACCCCCCGGAGGCGTCTCGCACAATAATACACAG68250.1835542887694897No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC67040.18030006621401598No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA66500.178847768544631No Hit
TCATTATAGTGGGAGCACCTACTACAACGCGTCCCTCAAGAGTCGAGTCA63710.17134423058614195No Hit
ATTCATTATAGTGGGAGCACCTACTACAACGCGTCCCTCAAGAGTCGAGT63040.16954230570005321No Hit
GGATTGGGAGGATTCATTATAGTGGGAGCACCTACTACAACGCGTCCCTC62690.16860100165508146No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC60080.16158156291972073No Hit
ATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGAACT59690.16053268126960937No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT58500.15733224751670546No Hit
GTAGTAGGTGCTCCCACTATAATGAATCCTCCCAATCCACTCCAGTCCCT56890.15300224890983544No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC56300.15141547923402593No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA54830.1474620022451446No Hit
CTACATAACCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCA53910.1449877173269332No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT53710.14444982930123504No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA51860.13947436506352726No Hit
GCCTACTATTATATGGATGTCTGGGGCAAAGGGACCACGGTCACCGTCTC51850.13944747066224233No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG51760.13920542105067818No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG51250.13783380658514793No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT50340.1353864160682214No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC50160.1349023168450931No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG48380.13011511341637966No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT48290.1298730638048155No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA48130.12944275338425698No Hit
CAGTAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGA48030.12917380937140793No Hit
GTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTACCGACT47040.12651126364420215No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT44680.12016418494096409No Hit
GCTGTGTATTATTGTGCGAGACGCCTCCGGGGGGTTCAGGGAGCCTACTA44140.11871188727157914No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG43520.11704443439191489No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42700.11483909348655252No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42160.11338679581716757No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT41520.11166555413493352No Hit
CATCTATGGTGCATTCAGTAGGGCCACTGGCATCCCAGACAGGTTCAGTG41250.11093940530024103No Hit
CAGTAGTACCGACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGAC40930.110078784459124No Hit
CTCCTGCAGGGCCAGTCCGAGTGTTAGCAGCAACTACATAACCTGGTACC40370.10857269798716922No Hit
ACCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGG40250.10824996517175033No Hit
TCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCAGTT39670.10669008989722573No Hit
GTGTATTACTGTCAGCAGTATGGTAGTTCTTTCGGCGGAGGGACCAAGGT39370.10588325785867853No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA39220.10547984183940493No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT39080.10510332022141623No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGAAGCCACCCTCTCCTGC38770.10426959378158412No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38580.10375860015717088No Hit
GTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGAC38140.10257524650063499No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG38140.10257524650063499No Hit
ACTACAACGCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCC37620.10117673763381982No Hit
CCCTGAACCCCCCGGAGGCGTCTCGCACAATAATACACAGCCGTGTCTGC37310.10034301119398771No Hit
GTCTACGGATATGGTGACTCGACTCTTGAGGGACGCGTTGTAGTAGGTGC37220.10010096158242353No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)