FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2785560
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT112960.4055198954608768No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC107460.38577521216559685No Hit
GTACTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT78580.28209767515329054No Hit
TTATTGTACTACGTGGGTGGCGTACTTTAATACTACCGGCTACTACACGT76930.2761742701647066No Hit
CTTTAATACTACCGGCTACTACACGTTTGACTCGTGGGGCCAGGGAACCC67920.24382888898462068No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT62200.22329441835752953No Hit
TTATTACTGCATGCAAACTCTACAAACTCCGTACACTTTTGGCCAGGGGA60030.21550424331193727No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT55810.2003546863108316No Hit
GTACAATAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTCCATTTGCAG53810.19317480147618432No Hit
GTATTATTGTACTACGTGGGTGGCGTACTTTAATACTACCGGCTACTACA52430.18822068094027772No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA51130.183553755797757No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC50970.18297936501098522No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTGACGCCTGG49720.1784919369893307No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49650.17824064102011802No Hit
GTATTAAAGTACGCCACCCACGTAGTACAATAATACACGGCTGTGTCCTC49070.1761584744180703No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT48260.1732506210600382No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC47050.1689067907350766No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAA45880.16470655810680795No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG45720.1641321673200362No Hit
CTACAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG45280.1625525926564138No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA44650.16029092893349992No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACA44580.16003963296428725No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42720.15336234006806532No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT42120.15120837461767114No Hit
CAATAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTCCATTTGCAGATC41700.14970059880239522No Hit
GTTTATTACTGCATGCAAACTCTACAAACTCCGTACACTTTTGGCCAGGG40610.14578756156751246No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC40090.1439207915105042No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG40000.14359769669294503No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG39350.14126423412168468No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA39160.1405821450623932No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG39080.1402949496690073No Hit
ATAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTCCATTTGCAGATCCA38400.13785378882522725No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT38200.1371358003417625No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA37620.13505363373971482No Hit
ATCCAGTACTTTGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC37080.13311506483436006No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG36600.13139189247404473No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC36050.1294174241445167No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGT35470.12733525754246902No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC35430.12719165984577607No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA34990.1256120851821537No Hit
ATACACGGCTGTGTCCTCGGTTTTCAGGCTGTCCATTTGCAGATCCAACA34480.12378121454931863No Hit
GGTCTAGTCAGAGCCTCCTGCAAAGTACTGGATACAACTATTTGGATTGG33620.1206938640704203No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT33520.12033486982868793No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGT33260.1194014848001838No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTA33170.11907838998262468No Hit
AGTCAGAGCCTCCTGCAAAGTACTGGATACAACTATTTGGATTGGTACCT33080.11875529516506556No Hit
CTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT32810.11778601071238817No Hit
CTCTACAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA32440.11645773201797843No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA32220.11566794468616723No Hit
CAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCC31960.11473455965766309No Hit
GTGTATTATTGTACTACGTGGGTGGCGTACTTTAATACTACCGGCTACTA31880.1144473642642772No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG31550.1132626832665604No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC30910.11096512011947329No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT30640.10999583566679591No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG30530.10960094200089031No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30060.1079136690647482No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30010.10773417194388203No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC30010.10773417194388203No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA29860.10719568058128348No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG29360.10540070937262166No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA29230.1049340168583696No Hit
GAGTTTGTAGAGTTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCC28900.1037493358606528No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA28670.10292364910466836No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC28550.10249285601458952No Hit
GCCGTGTATTATTGTACTACGTGGGTGGCGTACTTTAATACTACCGGCTA28230.10134407444104597No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28220.10130817501687274No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC28200.10123637616852627No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA28030.10062608595758124No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)