FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2785560
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT107770.3868880943149672No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC88130.3163816252387312No Hit
TTATTGTACTACGTGGGTGGCGTACTTTAATACTACCGGCTACTACACGT78730.2826361665158891No Hit
GTACTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT70770.254060224873993No Hit
CTTTAATACTACCGGCTACTACACGTTTGACTCGTGGGGCCAGGGAACCC66950.24034664483981674No Hit
TTATTACTGCATGCAAACTCTACAAACTCCGTACACTTTTGGCCAGGGGA65760.23607461336320165No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTGACGCCTGG65720.2359310156665087No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT64660.23212567670414566No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT58810.21112451356280243No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA54640.19615445368256296No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT53060.1904823446631916No Hit
GTATTATTGTACTACGTGGGTGGCGTACTTTAATACTACCGGCTACTACA51180.1837332529186232No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49330.1770918594465745No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG47890.17192234236562845No Hit
CTACAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG47770.1714915492755496No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC47170.16933758382515546No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA46450.16675282528468244No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAA46030.16524504946940652No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC45160.16212179956633496No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42080.1510647769209782No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA41490.14894671089475725No Hit
GTTTATTACTGCATGCAAACTCTACAAACTCCGTACACTTTTGGCCAGGG41260.14812102413877282No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACA39710.1425566133919212No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT39590.14212582030184237No Hit
GTACAATAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTCCATTTGCAG39520.1418745243326297No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA39400.14144373124255089No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG38840.13943336348884963No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG38420.1379255876735737No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC37520.13469463949798247No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37070.13307916541018683No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA36890.13243297577506857No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT36760.1319662832608165No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT35910.1289148322060914No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA35820.12859173738853227No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTGACGCCTGGATGAGCTGGG34740.12471459957782277No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGT34620.12428380648774394No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT34530.1239607116701848No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34300.12313502491420036No Hit
CTCTACAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA33900.12169904794727092No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC33420.11997587558695559No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33280.11947328364853027No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTA32840.11789370898490788No Hit
ATCCAGTACTTTGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC32540.1168167262597108No Hit
CTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT32130.1153448498686081No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGT32040.11502175505104899No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT31840.11430376656758426No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG31230.11211390169301684No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC30790.11053432702939445No Hit
GTATTAAAGTACGCCACCCACGTAGTACAATAATACACGGCTGTGTCCTC30390.109098350062465No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA30250.1085957581240397No Hit
CAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCC30200.1084162610031735No Hit
GTGTATTATTGTACTACGTGGGTGGCGTACTTTAATACTACCGGCTACTA30100.10805726676144113No Hit
CAATAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTCCATTTGCAGATC30090.1080213673372679No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG29870.10723158000545673No Hit
GGTCTAGTCAGAGCCTCCTGCAAAGTACTGGATACAACTATTTGGATTGG29710.10665718921868492No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC29370.10543660879679491No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG29160.10468272088915694No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGT29140.10461092204081046No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG29060.10432372664742456No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC29030.10421602837490486No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA28890.10371343643647957No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA28810.10342624104309366No Hit
ATAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTCCATTTGCAGATCCA28730.10313904564970779No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT28140.10102097962348684No Hit
GAGTTTGTAGAGTTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCC27970.10041068941254183No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)