FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3264651
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC141990.4349316358777707No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT141670.4339514392196899No Hit
GATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG75650.23172461619940388No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT70990.2174505023661028No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTACTAC69390.21254951907569908No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC66710.20434037206427275No Hit
AGTCAGAACATTACTACCTATTTAAATTGGTATCAGCAGAAATCAGGGAA66100.20247187218480628No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG65220.1997763313750842No Hit
GAGTTACAATAGCCAAGCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGA64670.19809161836900788No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG62370.19104645488905245No Hit
CATTACTACCTATTTAAATTGGTATCAGCAGAAATCAGGGAAAGCCCCTA59370.18185711121954537No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA53470.16378473533618143No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC52720.16148739941880466No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT52490.1607828830708091No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG52160.15977205526716332No Hit
GTAATACGTGGCTGTGTCCTCAGGGTCCATGTTGGTCATTGTAAGGACCA52110.1596188995393382No Hit
TTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT49790.15251247376825272No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT49510.15165480169243206No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA49270.1509196541988715No Hit
GTATTACTGTGCACGGACTCTAGAGATTTTAGATGATTACTTTTGGGGGA48110.14736644131332877No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC47270.14479342508586676No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA47260.14476279394030173No Hit
CAGTAATACGTGGCTGTGTCCTCAGGGTCCATGTTGGTCATTGTAAGGAC46270.1417303105293644No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG46120.14127084334588905No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCC46000.14090326959910876No Hit
ATTTAAATTGGTATCAGCAGAAATCAGGGAAAGCCCCTAAGCTCCTGATC45940.14071948272571863No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT45610.13970865492207285No Hit
ACTTACTACTGTCAACAGAGTTACAATAGCCAAGCGCTCACTTTCGGCGG45390.13903476971964232No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG45280.13869782711842704No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC45150.13829962222608175No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC43960.13465451590384392No Hit
GAACATTACTACCTATTTAAATTGGTATCAGCAGAAATCAGGGAAAGCCC42860.13128508989169133No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA42820.13116256530943124No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG42800.1311013030183012No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41670.12763998356945352No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT41350.12665978691137275No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG41150.1260471640000723No Hit
CTAGTGGAATGTGTGTGAGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTG40810.12500570505086148No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC40790.12494444275973145No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA40490.12402550839278072No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT40240.12325972975365514No Hit
TCTCTGAAGACCAGGCTCACCATCTCCAAGGACACCTCCAAAAACCAGGT39440.12080923810845325No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC39110.11979841030480746No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC38770.11875695135559668No Hit
CTCTAGAGATTTTAGATGATTACTTTTGGGGGAGTTATCGTACATACGGT38630.11832811531768633No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG38230.11710286949508537No Hit
TCTCTAGAGTCCGTGCACAGTAATACGTGGCTGTGTCCTCAGGGTCCATG37250.11410101722971305No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG37150.11379470577406282No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA37110.11367218119180274No Hit
TCCATACCGTATGTACGATAACTCCCCCAAAAGTAATCATCTAAAATCTC36720.11247756651476683No Hit
AGTGGAATGTGTGTGAGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTGGA36400.11149736985668607No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGATTTCTGCTGATACCAATT35710.10938382081269943No Hit
ACGTATTACTGTGCACGGACTCTAGAGATTTTAGATGATTACTTTTGGGG35040.10733153405984285No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC35030.10730090291427782No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35010.10723964062314777No Hit
CCCTCACACTGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGA34470.10558555876263649No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC34380.10530987845255128No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC34320.10512609157916114No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG34140.10457473095899072No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA34100.10445220637673062No Hit
GCTACTGACTGTCCCGTCCTGGGTCTTATCCCAGGTCACCTTGAGGGAGT34100.10445220637673062No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT34010.10417652606664542No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG33950.10399273919325527No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA33880.1037783211743001No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG33250.10184855900370361No Hit
CTCCTGATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT33240.10181792785813859No Hit
ACTGTCAACAGAGTTACAATAGCCAAGCGCTCACTTTCGGCGGAGGGACC33090.10135846067466324No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGA32980.10102151807344797No Hit
GCACTAGTGGAATGTGTGTGAGCTGGATCCGTCAGCCCCCAGGGAAGGCC32880.10071520661779775No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC32850.10062331318110268No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC32770.10037826401658248No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)