FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3881599
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG174250.4489129351074132No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC138240.3561418889483432No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT122170.3147414248612492No Hit
GTATAGCAGCGGGGTCGGACTACTGGGGCCAGGGAACCCTGGTCACCGTC118290.30474554429759493No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG117280.3021435238415921No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT110850.285578185691No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT110420.2844703948037909No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACT109540.2822032878718281No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA108570.27970431773091453No Hit
TCCTATGACAGCAGCCTGAGTGCCCTTTGGGTGTTCGGCGGAGGGACCAA107560.2771022972749117No Hit
ATGTATTACTGTGCGAGACATCGGGGCAGTATAGCAGCGGGGTCGGACTA103020.2654060865122853No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT102570.26424677046753153No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC101500.2614901745388949No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG101220.26076882233327037No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACT99500.25633765878443393No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA98040.25257632228367743No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGCCCTTTGGGTGTT97370.250850229505933No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG96140.24768143231693948No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT88440.22784424666226472No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGGGCACTC88360.22763814603208626No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC87250.2247784997883604No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCC86490.22282054380166524No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCT81650.2103514556758697No Hit
CTGCTATACTGCCCCGATGTCTCGCACAGTAATACATGGCGGTGTCCGAG81450.20983620410042356No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA81320.20950129057638361No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA78580.20244234399277206No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC77570.19984032353676925No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG77340.19924778422500625No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA76540.19718677792322187No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG75550.19463628262476365No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT73980.1905915577575118No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG73550.18948376687030266No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA73220.18863360177081662No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA72450.18664988320534912No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA72180.1859542935784969No Hit
CTCCTCATCTATGGTAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATT71210.18345532343758333No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA69920.18013195077595598No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC69620.17935907341278684No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG68820.17729806711100246No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA68440.17631908911765484No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG66350.17093471015424314No Hit
GTATAAGAGTCACTAGGATCAATCCTCCCCATCCACTCCAGGCCTTTCCC66090.1702648831061632No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC65520.16879641611614182No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA64610.16645202144786206No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC64030.1649577918790684No Hit
GTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTC63630.1639272887281762No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC62800.1617889946900749No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT61760.15910968649775517No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA61150.1575381691926446No Hit
CTCTTATACCAACTACAGCCCGTCCTTCCAAGGCCACGTCACCATCTCAG60750.15650766604175237No Hit
GTCCTATGACAGCAGCCTGAGTGCCCTTTGGGTGTTCGGCGGAGGGACCA60700.15637885314789085No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGGGCACTCA58830.15156125091746986No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA58760.1513809128660637No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT57600.14839245372847634No Hit
CCCAAAGGGCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCC56920.1466405983719596No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC56620.14586772100879045No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC56090.1445023043338583No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT55600.14323993797401535No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGT54130.1394528388944865No Hit
CCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCAGCTGGGTG53990.13909216279167425No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG53630.13816470995587127No Hit
ATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTC53250.13718573196252368No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC52830.1361037036540869No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT52290.13471252440038242No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT51810.13347592061931177No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC51010.1314149143175274No Hit
GTATTACTGTGCGAGACATCGGGGCAGTATAGCAGCGGGGTCGGACTACT50970.13131186400243816No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT50950.13126033884489358No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT50930.13120881368734894No Hit
CTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTAGACGGGGTACGTGCC50550.13022983569400137No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG50470.13002373506382292No Hit
GTCTGGAGCAGAGGTGAAAAAGCCCGGGGAGTCTCTGAGGATCTCCTGTA49980.12876136870397997No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT48860.12587595988148184No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT48510.12497426962445116No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGG48000.12366037810706361No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47990.1236346155282913No Hit
GGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAAC47930.12348004005565748No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC45570.11740007146539351No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT45290.11667871925976898No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT44820.11546787805747066No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC44680.11510720195465837No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG44540.11474652585184611No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG44280.11407669880376617No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC43810.11286585760146785No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC43800.11284009502269554No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGA42940.11062451324827732No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA42310.10900147078562211No Hit
CCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC42040.10830588115876988No Hit
ATTACTGCCAGTCCTATGACAGCAGCCTGAGTGCCCTTTGGGTGTTCGGC41930.10802249279227452No Hit
CATCTATGGTAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTG41330.10647673806593622No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA41120.10593572391171782No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT40560.10449301950046874No Hit
TCTCCAAGGAGTCTGTGCCGAAGTGCAGCTGGTGCAGTCTGGAGCAGAGG40280.10377166729484422No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG40210.10359132924343809No Hit
CAGTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTA40030.1031276028255366No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC39930.10286997703781353No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG39610.10204557451709978No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT39270.1011696468388414No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG39210.10101507136620759No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)