FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3961028
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG137800.34788948727451563No Hit
GTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA128010.3231736811756948No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG123760.31244414328805553No Hit
GTATATTACTGTGCGAGCGATTATTATGATAGAAGTGCTCATTTCTCCTC105620.2666479509864611No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT100450.25359578372079167No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC90770.2291576833084745No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT87120.21994290371085487No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT79970.20189203408812056No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC69240.17480310666826895No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG66690.16836538393568537No Hit
ATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTG63650.16069060859958578No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA62880.15874666879406055No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT56820.14344761006486195No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT54690.13807021813529216No Hit
GTATGGTAACTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA53880.1360252944437656No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG52970.1337279110372358No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT50180.12668428498864437No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC49050.12383149020910734No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC48820.1232508328645998No Hit
AGCAAATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA48480.12239246983358865No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT47740.1205242679425644No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG47450.11979213476905491No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC46770.11807540870703262No Hit
GCTGTATATTACTGTGCGAGCGATTATTATGATAGAAGTGCTCATTTCTC45710.11539933572799789No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTG44830.11317769023596903No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43790.11055210919993497No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42080.10623504807337893No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG41430.10459405992585763No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA41240.1041143864673514No Hit
GTTTTACTGTCAGCAGTATGGTAACTCACCGTGGACGTTCGGCCAAGGGA41220.10406389452435076No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT40530.10232192249082814No Hit
ATATTACTGTGCGAGCGATTATTATGATAGAAGTGCTCATTTCTCCTCGT40210.10151405140281765No Hit
TGATAGAAGTGCTCATTTCTCCTCGTTAGTCTTCCAGCACTGGGGCCAGG39750.10035273671380258No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)