FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4004218
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATACA203600.5084638248966464No Hit
GTATATTACTGTGCGAAATTGAGTACTAAGCTAGTAGTGCCCCAGGTAGG185620.46356117474123537No Hit
CTACTAGCTTAGTACTCAATTTCGCACAGTAATATACGGCCGTGTCCTCG118850.2968120117336269No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA114830.2867725982950978No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGTTGTTGGAGTCTGGGGGAGGCT108690.2714387678193345No Hit
AGCTAGTAGTGCCCCAGGTAGGGCCCTCCCCCTTCTTTGACTACTGGGGC106460.2658696404641306No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATACAGCG85630.21384949570677722No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT84230.210353182568981No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA80440.20088816343166133No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC79050.19741682395913507No Hit
GCCGTATATTACTGTGCGAAATTGAGTACTAAGCTAGTAGTGCCCCAGGT77940.1946447471141681No Hit
GGTATAAGTGGTAGTGGTGGTAGCACATACTACGCAGACTCCGTGAAGGG77390.19327119552431962No Hit
ATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT74600.1863035429139972No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC72640.18140870452108251No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC71170.1777375757263965No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA70480.1760143928227684No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG63370.15825811681581772No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT63350.15820816948527777No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA60610.1513653852013052No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT56610.1413759190933161No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG56300.14060173546994692No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA55400.1383541055956494No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC54910.13713039599742072No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA54040.13495768711893308No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT53690.13408360883448403No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC53680.13405863516921407No Hit
ATATTACTGTGCGAAATTGAGTACTAAGCTAGTAGTGCCCCAGGTAGGGC52250.13048740103560794No Hit
GTGTGAGGTGCAGTTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGG51790.1293386124331892No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGG51660.12901395478467956No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA51490.12858940247509001No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA51460.1285144814792801No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCC51160.12776527152118092No Hit
GTATTTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTATATTACT51110.12764040319483105No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT49850.12449372137081446No Hit
GTATGTGCTACCACCACTACCACTTATACCTGAGACCCACTCCAGCCCCT48870.12204630217435714No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGCAGTGCTGCTG48770.12179656552165741No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA48690.12159677619949763No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC48120.12017327727910918No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT47150.11775083174792181No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG45980.114828912911335No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG45380.11333049299513663No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA44510.11115778411664899No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC44030.10995904818369029No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG43200.10788623396628255No Hit
CTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCA42550.10626294572373433No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG42290.10561363042671504No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG42140.10523902544766545No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41460.10354081620930729No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA41070.10256684326377834No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT40540.10124323900446978No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)