FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4550539
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGATCTCAGGCTGCTCAGCTCCATGTACA127230.2795932525795296No Hit
GTATATTACTGTGCCAGAGGTGACGCAGTGGCTGGTCCTAGAAATCTTAA97290.21379884888361578No Hit
GTGTACATGGAGCTGAGCAGCCTGAGATCCGAGGACACGGCCGTATATTA78720.17299049629065918No Hit
CTATATCCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG75980.166969231556965No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC66960.14714740385699365No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT62590.1375441458693135No Hit
CTACAGAAGCCTCTGAGAGGAAAGTTCTTCACCATGGACTGGACCTGGAG61220.13453351350246642No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGCTTCCTGCA58370.128270519162675No Hit
ATATACGGCCGTGTCCTCGGATCTCAGGCTGCTCAGCTCCATGTACACTG51030.11214056181037015No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50620.11123956964218965No Hit
ACTATATCCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG49910.10967931491192581No Hit
GTGATGGAAACACCTACTTGAGTTGGCTTCAGCAGAGGCCAGGCCAGCCT49840.10952548698077305No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT48940.10754769929452314No Hit
TCTGGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG48600.1068005350574954No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT48400.10636102668277318No Hit
CCATGTACACTGTGTTCGTGGACGTGTCCCTGGTCATGGTGACTCTGCCC47520.1044271898339955No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGG47300.10394373062180107No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)