FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4550539
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGATCTCAGGCTGCTCAGCTCCATGTACA168920.37120877329037283No Hit
GTATATTACTGTGCCAGAGGTGACGCAGTGGCTGGTCCTAGAAATCTTAA94100.20678869030679664No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG89700.19711950606290815No Hit
GTGTACATGGAGCTGAGCAGCCTGAGATCCGAGGACACGGCCGTATATTA79580.17488038230196468No Hit
ATATACGGCCGTGTCCTCGGATCTCAGGCTGCTCAGCTCCATGTACACTG72410.15912400706817367No Hit
CCATGTACACTGTGTTCGTGGACGTGTCCCTGGTCATGGTGACTCTGCCC68240.14996025745521574No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT65530.1440049189777299No Hit
CTATATCCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG64280.14125799163571612No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGG56180.12345790245946689No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGCTTCCTGCA55370.12167789354184197No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54710.1202275159052587No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG53910.11846948240636988No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47780.10499855072113434No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)