FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3509057
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG230920.6580685352218559No Hit
GTAATACACGGCCGTGTCTTCAGATCTCAGGCTGCTCAGGTCCATGTAGA170760.4866264640329296No Hit
GTACTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGA142560.4062629931631204No Hit
GTGTATATAGTACCTGGTGAAGGTGTATCCAGATGCCTTGCAGGAAACCC120360.34299813311667493No Hit
GTGTATTACTGTGCGAGAGATTATGGTTCGGGGAGTTATACAACCGACTA99310.28301050681137413No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT93470.2663678589433002No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT91990.26215020160687047No Hit
GTCTACATGGACCTGAGCAGCCTGAGATCTGAAGACACGGCCGTGTATTA90490.25787554890102954No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA86830.24744539629877768No Hit
ATATAGTACCTGGTGAAGGTGTATCCAGATGCCTTGCAGGAAACCCTCAC84100.23966552837414723No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC81450.23211364192716163No Hit
ATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGA77620.22119902868491448No Hit
GATTATTACTGCTACTCATATGCAGGCAGCTACACTTCTGTCTTCGGAAC77240.22011611666610145No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA76860.21903320464728843No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAGAAGTGTAGCTGC74320.2117947927320645No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC72800.20746314465681237No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT69900.19919881609218656No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC68610.1955226147651634No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC68420.1949811587557569No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA67640.1927583393487196No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66540.18962359403110293No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAGGGTTTCCTGCA66430.1893101194993413No Hit
TTATAACTATGTCTCCTGGTACCAACAGCACCCAGCCAAAGCCCCCAAAC65990.1880562213722946No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT65540.18677382556054234No Hit
ACTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG65320.186146876497019No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA65070.18543443437937884No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA64680.1843230246758602No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC61980.17662864980534657No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG61970.17660015212064095No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT61560.1754317470477111No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC61480.17520376557006626No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG60750.17312343458655702No Hit
CATGTAGACTGTGGTCGTGGACGTGTCCCTGGTCATGGTGACTCTGCCCT60670.17289545310891216No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA60560.1725819785771505No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAGAAGTGTAGCTG59950.17084361981010854No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT56510.16104041627138002No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC54720.15593933070907653No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT54700.15588233533966533No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA54370.15494191174438032No Hit
CTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGG54080.15411547888791777No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG53150.1514651942102964No Hit
GTTGTATAACTCCCCGAACCATAATCTCTCGCACAGTAATACACGGCCGT51590.14701955539622183No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC51130.14570866189976397No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT50830.14485373135859578No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG49900.1422034466809744No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC49260.14037959485981563No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA48530.13829926387630637No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTACTCATAT48220.13741583565043258No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT46970.13385362506223183No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG46440.13234324777283468No Hit
GTATTACTGTGCGAGAGATTATGGTTCGGGGAGTTATACAACCGACTACT45570.12986394920344696No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC45410.12940798624815728No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT45390.12935099087874607No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTAATTATAACTATGTCTCCT45270.1290090186622788No Hit
ATACACGGCCGTGTCTTCAGATCTCAGGCTGCTCAGGTCCATGTAGACTG45130.1286100510764003No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG45020.12829657654463864No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC44970.12815408812111062No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT44310.1262732409305406No Hit
CAGTAATACACGGCCGTGTCTTCAGATCTCAGGCTGCTCAGGTCCATGTA44300.12624474324583498No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG43640.12436389605526499No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG42780.12191309517058287No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA42510.1211436576835315No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT42080.11991825724119043No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC41730.11892083827649423No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC41710.11886384290708302No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATATTCAAC41110.11715398182474664No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG39860.11359177123654589No Hit
ACATGGACCTGAGCAGCCTGAGATCTGAAGACACGGCCGTGTATTACTGT39820.11347778049772346No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA38750.11042852823422361No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT38340.10926012316129374No Hit
GTATAACTCCCCGAACCATAATCTCTCGCACAGTAATACACGGCCGTGTC37790.10769275050248543No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTG37670.10735077828601815No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTC36470.10393105612134543No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT36450.10387406075193421No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG36410.10376007001311179No Hit
GGAATATTCAACCCTAGTGGTGGTAGCACAAGCTACGCACAGAAGTTCCA36330.10353208853546693No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG36180.10310462326488284No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAATATTCAACCCTAGTGGTGGT36130.10296213484135482No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT35890.10227819040842026No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG35760.10190772050724739No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT35590.10142325986725209No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG35350.10073931543431755No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)