FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3380532
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA105290.31145985306454727No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT101440.3000711130674107No Hit
CTATTACACTGGGAGCACCAACTACAATCCCTCCCTCAAGAGTCGAGTCA98850.292409597069337No Hit
GTGTATTACTGTGCGAGGGCCGCCACAGTGACTAAGGTTATCTACTATTA93710.2772048896445885No Hit
GGATATATCTATTACACTGGGAGCACCAACTACAATCCCTCCCTCAAGAG86230.2550781947930089No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT81610.24141170679644502No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA80770.2389268907970698No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC79170.23419390794111694No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC74910.22159234108714249No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC72910.21567611251720142No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT72410.21419705537471617No Hit
ATCTATTACACTGGGAGCACCAACTACAATCCCTCCCTCAAGAGTCGAGT72040.21310255308927706No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA70650.20899077423316803No Hit
TCATTAGACACGTCCAAGAACTTGTTCTCCCTGAAGCTGAGCTCTGTGAC69500.20558894280545192No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69460.2054706182340531No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA68150.20159548852074172No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATACAGAGTGCTGC64210.1899405182379578No Hit
GACTAAGGTTATCTACTATTACGGTATGGACGTCTGGGGCCAAGGGACCA64110.18964470680946074No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA62180.18393554623946765No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC61530.1820127719542368No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC60770.17976460509765918No Hit
TCTCTGGGCTCCGGGCTGAGGACGAGGCTGATTATTACTGCAGTTCATAT60290.17834471024087334No Hit
CTATTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT59830.17698397766978688No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT59540.17612612452714543No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA59510.17603738109859632No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT59380.17565282624155015No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC59260.17529785252735366No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA57490.17006199024295585No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC57350.16964785424305998No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT56840.168139215957725No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATGACTATGTCTCCT56230.16633476624389298No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG55880.16529942624415328No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC55240.16340623310177213No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT54930.1624892176734313No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT53060.1569575439605364No Hit
GATTATTACTGCAGTTCATATGCAAGCAGCAGCACTCTGTATGTCTTCGG51420.15210623653318472No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATACAGAGTGCTG50900.15056801710500003No Hit
GGATTGGATATATCTATTACACTGGGAGCACCAACTACAATCCCTCCCTC49750.14716618567728393No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA49180.1454800605348507No Hit
GTATTACTGTGCGAGGGCCGCCACAGTGACTAAGGTTATCTACTATTACG48500.14346854282107074No Hit
CTACAATCCCTCCCTCAAGAGTCGAGTCACCATATCATTAGACACGTCCA47070.13923843939356292No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT46930.13882430339366703No Hit
CTGTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAA46710.13817351825097351No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG45970.13598451368009534No Hit
ACCTTAGTCACTGTGGCGGCCCTCGCACAGTAATACACGGCCGTGTCTGC45780.13542247196595092No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC45300.13400257710916508No Hit
GGTTATGACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAA43860.1297428925388075No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC43220.12784969939642635No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG43140.12761305025362873No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC42550.1258677628254961No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCC42030.12432954339731143No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC41910.12397456968311496No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT41870.12385624511171615No Hit
CCGTAATAGTAGATAACCTTAGTCACTGTGGCGGCCCTCGCACAGTAATA41510.12279132396912674No Hit
GTGTAATAGATATATCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCG41480.12270258054057764No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT40540.11992195311270534No Hit
GATATATCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGATCCAGC40430.11959656054135859No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG40210.11894577539866505No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATG39950.11817666568457272No Hit
GTTCATATGCAAGCAGCAGCACTCTGTATGTCTTCGGAACTGGGACCAAG39260.11613556682794304No Hit
TTTATGGGGTCACTAATCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGC38970.11527771368530161No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC38750.11462692854260809No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGT38340.11341410168577017No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA37640.1113434216862908No Hit
GACATACAGAGTGCTGCTGCTTGCATATGAACTGCAGTAATAATCAGCCT36690.1085332131155688No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC36320.1074387108301297No Hit
GTCTAATGATATGGTGACTCGACTCTTGAGGGAGGGATTGTAGTTGGTGC36230.10717248054448235No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT36180.10702457483023381No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCT35390.1046876645451071No Hit
GTCTCTGGTGGCTCCATCAGGAGTCACTACTGGAGCTGGATCCGGCAGCC34920.10329735083117095No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG34840.10306070168837331No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA34170.10107876511744306No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG33860.10016174968910219No Hit
GTAATAATCAGCCTCGTCCTCAGCCCGGAGCCCAGAGATGGTCAGGGAGG33840.10010258740340279No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)