FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4630218
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATACA261970.565783295732512No Hit
GTATATTACTGTGCGAAAGATGGGTACGTGACTACAATAACTACACGAGG213590.4612957748425668No Hit
ATATTATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCA192900.416611053734403No Hit
TTATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC168430.3637625701424857No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT150420.32486591344079263No Hit
AGCACATACTACGCAGACTTTGTGAAGGGCCGGTTCACCATCTCCAGAGA131000.282924043749128No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG129650.2800084142906446No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT128490.2775031326818737No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT125660.27139110944668265No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC124460.26879943881691964No Hit
ATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA117110.25292545620962126No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATACAGCG110290.23819612813046814No Hit
GTTTTATACAGCTCCAACAATAAGAACTCCTTAGCTTGGTACCAGCAGAA110160.23791536381224382No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA109380.23623077790289787No Hit
GTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAG107810.23284000882895797No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC96980.20945018139534682No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC89830.19400814389300894No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA89580.19346821251180832No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT89350.19297147564110373No Hit
GCCGTATATTACTGTGCGAAAGATGGGTACGTGACTACAATAACTACACG85520.18469972688111014No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT80590.1740522800438338No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA74370.16061878727956222No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT73790.15936614647517675No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC72850.1573360044818624No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT72550.15668808682442167No Hit
TTATTACTGTCAGCAATATTATAGTACTCCGTACACTTTTGGCCAGGGGA71730.1549171118940836No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGG69840.15083523065220686No Hit
ATATTACTGTGCGAAAGATGGGTACGTGACTACAATAACTACACGAGGTC65250.14092209049336338No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT64100.13843840613984051No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT63870.13794166926913592No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGG62970.13599791629681368No Hit
GAGTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGC62880.13580354099958145No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA62170.13427013587697165No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC62130.13418374685597956No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA61200.13217520211791325No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCGTACACTTTTGGCCAGGG60890.13150568720522446No Hit
GTATGTGCTACCACCACTACCACTAATAGCTGAGACCCACTCCAGCCCCT60060.12971311501963836No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG59820.1291947808936858No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA59710.1289572110859575No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC58410.12614956790371426No Hit
GTATTTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTATATTACT58360.12604158162747414No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT58190.1256744282882577No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCC57860.12496171886507287No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCT57720.12465935729160052No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC57400.12396824512366372No Hit
AAGTAGTACAGCCGACCTCGTGTAGTTATTGTAGTCACGTACCCATCTTT56310.12161414430162899No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT55470.1197999748607949No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC55040.11887129288512982No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG54190.11703552618904768No Hit
ATACTACGCAGACTTTGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT54090.11681955363656744No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG54020.11666837284983127No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA53860.11632281676586285No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG53830.11625802500011878No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA52120.1125648943527065No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT51890.11206815748200191No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT51840.1119601712057618No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATA51750.11176579590852957No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT51530.11129065629307304No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC51490.11120426727208094No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC50920.1099732237229435No Hit
TGCAAATACAGCGTGTTCTTGGAATTGTCTCTGGAGATGGTGAACCGGCC50110.10822384604785348No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG50040.10807266526111729No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT48660.10509224403688984No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48320.104357937358457No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGGAGTTCTTA47530.102651754193863No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCA47300.10215501732315845No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT47290.1021334200679104No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA47260.10206862830216634No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)