FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328744
Sequences flagged as poor quality0
Sequence length101
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55061.674859465115713No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43321.3177426812352468No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25900.7878470785778601No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25720.7823716934757745No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA25350.7711167352103764No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA21600.6570462122502616No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA20570.6257148419438834No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA17850.5429756892901467No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA16310.49613072786119294No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15720.4781836322488015No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG14570.44320200520769965No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA13720.41734602000340687No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA13690.41643345581972596No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA13060.39726960796242666Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12690.3860146496970287No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG12280.3735429391867228No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA11840.360158664492736No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11470.348903706227338No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA10410.31665977173727883No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT10000.30418806122697295No Hit
GTATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA9660.2938456671452559No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC9490.28867447010439734Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA9480.2883702820431704No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA9190.27954882826758815No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8660.2634268610225586No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA8610.2619059207164237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT8090.24608814153262112No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT8010.24365463704280535No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT7970.24243788479789743No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7740.23544155938967706No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATC7590.2308787384712725Clontech Universal Primer Mix Long (96% over 26bp)
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA7540.22935779816513763No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7200.21901540408342055No Hit
TATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA7100.21597352347115079No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA6990.2126274547976541No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA6810.20715206969556857No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA6550.1992431801036673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG6390.1943761711240357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT6320.19224685469544692No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA6270.19072591438931205Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAA6170.18768403377704232No Hit
GTACGGGAAGCAGCGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5870.17855839194023312No Hit
GAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5860.17825420387900615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG5830.17734163969532524No Hit
GAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5710.17369138296060158No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCA5640.17156206653201275Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA5630.17125787847078577No Hit
GAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA5490.16699924561360815No Hit
GTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5460.16608668142992722No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG5220.1587861679604799No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACG4910.14935633806244372Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT4900.14905215000121677No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT4830.14692283357262795No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4810.146314457450174No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAA4800.146010269388947No Hit
TATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA4740.1441851410215852No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG4680.14236001265422335Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAA4650.14144744847054241No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCG4450.13536368724600295Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4310.13110505438882536No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT4270.12988830214391744No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA4180.12715060959287472No Hit
GTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4100.1247171051030589No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT4080.12410872898060497No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGAT3890.11832915581729249Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA3870.11772077969483853No Hit
GTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA3740.11376633489888789No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT3610.10981189010293724No Hit
GTACGGGAAGTAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3570.10859513785802935No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT3500.10646582142944054No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAA3460.10524906918453264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT3440.1046406930620787No Hit
GTATCAACGCAGAGTACGGGAAGCAGCGGTATCAACGCAAAAAAAAAAAA3300.10038206020490108No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)