FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5690321
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC129310.2272455279763655No Hit
GTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCAATTGCAGATACA127290.2236956403689704No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG114830.20179880888969182No Hit
GTATATTACTGTGCGAGAGGGGGGTTCGGGGAGACCTTTGACTACTGGGG90220.1585499306629626No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT89220.15679256056029175No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCCGGAAGATCA87520.15380503138575136No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG86990.1528736252313358No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT86950.15280333042722896No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG85150.14964006424242146No Hit
TACATATACTACGCAGACTCAGTGGAGGGCCGATTCACCATCTCCAGAGA81780.14371772699642077No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA78960.138761943306889No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA74700.13127554666951124No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT69220.1216451585068751No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG69100.1214342740945546No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA69020.12129368448634092No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC67760.11907939815697569No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC67500.11862248193028127No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA63260.11117123269495693No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT62940.11060887426210227No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGC61890.10876363565429789No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCC60530.10637361231466555No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC60100.10561794317051709No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCCGGAAGATCAC59740.10498528993355559No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA59630.1047919792222618No Hit
CTAGTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC59570.10468653701610155No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA59520.10459866851096802No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT59120.10389572046989967No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG58850.10342123054217855No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)