FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534528
Sequences flagged as poor quality0
Sequence length101
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91221.7065523227969348No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA61421.149051125478927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52410.9804911997126436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49270.9217477849616857No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA48470.9067813098659003No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA35830.6703110033524904No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA33610.6287790349616859No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA29190.5460892600574713No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG26050.48734584530651337No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA24140.4516133860153257No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG22660.4239254070881226No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA20310.3799613864942529No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA19840.37116858237547895No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19170.3586341594827586No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18610.34815762691570884No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17420.325894995210728No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAA17180.3214050526819923No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA16490.30849646791187735No Hit
GTATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA15640.29259458812260536No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT15590.2916591834291188No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA15240.28511135057471265No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA14890.2785635177203065No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA14820.2772539511494253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG14260.26677741858237547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG13890.2598554238505747No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA13840.2589200191570881No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAA12760.2387152777777778No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAA12390.231793283045977No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA12230.22879998802681992No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT12130.22692917863984674No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG11950.22356172174329503No Hit
TATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA11620.21738805076628354No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT11580.21663972701149425No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA11530.21570432231800768No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT11170.20896940852490423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG10440.1953125No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA10180.19044839559386972No Hit
GAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA9860.18446180555555555No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA9750.18240391522988506No Hit
TATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA9400.17585608237547895No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG9100.1702436542145594No Hit
GTACGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAAAA8950.1674374401340996No Hit
GTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA8890.1663149545019157No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT8740.16350874042145594No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT8700.16276041666666669No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA8550.1599542025862069No Hit
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8130.15209680316091956No Hit
GTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7930.14835518438697318No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA7900.14779394157088122No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA7860.14704561781609196No Hit
GAGTACGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAA7690.14386524185823754No Hit
GTATCAACGCAGAGTACGGGAAGCAGCGGTATCAACGCAAAAAAAAAAAA7560.1414331896551724No Hit
GAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7510.14049778496168583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA7480.13993654214559387No Hit
GGTATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAA7450.13937529932950193No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7430.1390011374521073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT6950.130021252394636No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAA6800.12721503831417624No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT6740.12609255268199235No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6730.12590547174329503No Hit
TATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA6600.12347341954022989No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6600.12347341954022989No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT6550.12253801484674329No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6480.12122844827586207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA6470.12104136733716475No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT6260.11711266762452106No Hit
GAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6080.11374521072796935No Hit
TATCAACGCAGAGTACGGGAAGCAGCGGTATCAACGCAAAAAAAAAAAAA5940.1111260775862069No Hit
ATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5820.10888110632183909No Hit
GTACGGGAAGCAGCGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5800.10850694444444445No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT5710.10682321599616858No Hit
GGTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA5570.10420408285440612No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAA5420.10139786877394637No Hit
GTACGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAAAA5390.1008366259578544No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT5380.10064954501915709No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT5360.10027538314176246No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)