FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5828823
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGCTTT126390.21683622920098963No Hit
GTTCATATAGTGGTCACTGAAACTGAATCCAGAGGCTGCACAGGAGAGTC95740.16425271448455375No Hit
TATAAAAGGTGTCCAGTGTCAAGCGCAACTGGTGGAGTCTGGGGGAGGCT89700.15389041664157582No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT80140.1374891637642797No Hit
GTCTTAATTATATATAAAGACACTGAGAGGCCCTCAGGGATCCCTGAGCG77490.13294279136628442No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC73030.1252911608398471No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT65190.11184076099068371No Hit
ATAATAGGCAGCCTCGTCCTCTGCCTGGACTCCACTGATGGTCAACGTGA65020.11154910691232175No Hit
CTATTGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTTTT63770.10940459163024852No Hit
AATTAAGACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATATTCATATT63040.10815219470551773No Hit
GACTTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCGATAAGTACCA60840.10437784780906884No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA59380.10187305395960729No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG58720.10074074989067261No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)