FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3065152
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAATACTTACCCGCGCTTCGGCCAAGGGACACGACTGGAGATTAAAC109900.35854665608752845No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT92300.30112699141837007No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC92260.3009964921804857No Hit
GTATTAAAGTGTTGACAGTAATAAGTTGCCAAATCTTCAGGCTGCAGGGA78820.25714874825131023No Hit
TGATTATACCATGCACTGGGTCCGTCAAGGTCCGGGGAAAGGTCTGGAGT76190.2485684233604076No Hit
ACTTATTACTGTCAACACTTTAATACTTACCCGCGCTTCGGCCAAGGGAC56790.18527629298644896No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG52450.17111712567598605No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT49060.16005731526527886No Hit
ATACAAGGCGGTGTCCTCAGATCTCAGACTGTTCATTTGCAGATACAGGG47680.1555550915582653No Hit
CAATAATACAAGGCGGTGTCCTCAGATCTCAGACTGTTCATTTGCAGATA46690.15232523542062515No Hit
GTATAATCATCAAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGA46550.15186848808802958No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCG46270.1509549934228384No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46210.15075924456601175No Hit
TATTTAGCCTGGTATCAGCAAAAACCGGGGAAAGCCCCTAAGCTCCTGAT45130.14723576514313158No Hit
GTATTATTGTGCAAAAGATAAGGGCAGTGGCTGGTACCGCTGGTTCTTCG44230.14429953229073142No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAG42970.1401888062973712No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT42410.1383618169669889No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG42200.13767669596809556No Hit
CTCTTATTAGTTGGGATGGTGGTAGCACATCCTATGCAGACTCTGTGAAG41330.13483833754410873No Hit
TTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAGTCG40740.1329134737853131No Hit
ATAATACAAGGCGGTGTCCTCAGATCTCAGACTGTTCATTTGCAGATACA38910.12694313365209947No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG38270.12485514584594827No Hit
GTGGTAGCACATCCTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCC37960.12384377675234376No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC37890.12361540308604597No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA37460.12221253627878813No Hit
GTAATAAGTTGCCAAATCTTCAGGCTGCAGGGAGCTGATTGTGAGAGTGA36570.11930892823585909No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT36490.1190479297600902No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC35920.11718831562023678No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35810.11682944271605453No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT35350.11532870148038335No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA35210.11487195414778778No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34950.11402370910153885No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTT34860.11373008581629883No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC34630.11297971519846324No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT34400.11222934458062765No Hit
GTCTGAGATCTGAGGACACCGCCTTGTATTATTGTGCAAAAGATAAGGGC34250.11173997243856097No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT33660.10981510867976532No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33390.10893423882404527No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG33240.10844486668197859No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG33240.10844486668197859No Hit
ACCATGCACTGGGTCCGTCAAGGTCCGGGGAAAGGTCTGGAGTGGGTCTC32900.10733562315996074No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC31840.1038773933560228No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA31770.10364901968972502No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)