FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3065152
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAATACTTACCCGCGCTTCGGCCAAGGGACACGACTGGAGATTAAAC115680.3774037959618316No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT99680.3252041008080513No Hit
GTATTAAAGTGTTGACAGTAATAAGTTGCCAAATCTTCAGGCTGCAGGGA98270.3206040026726244No Hit
TGATTATACCATGCACTGGGTCCGTCAAGGTCCGGGGAAAGGTCTGGAGT76150.24843792412252313No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76130.2483726745035809No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC73370.2393682270895538No Hit
ACTTATTACTGTCAACACTTTAATACTTACCCGCGCTTCGGCCAAGGGAC62140.20273056605349424No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT54200.17682646733343077No Hit
GTAATAAGTTGCCAAATCTTCAGGCTGCAGGGAGCTGATTGTGAGAGTGA51950.16948588520243044No Hit
GTATAATCATCAAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGA50250.16393966759234124No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT49090.1601551896936922No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG48310.1576104545549454No Hit
CAATAATACAAGGCGGTGTCCTCAGATCTCAGACTGTTCATTTGCAGATA45830.14951950180610946No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG44770.1460612720021715No Hit
ATACAAGGCGGTGTCCTCAGATCTCAGACTGTTCATTTGCAGATACAGGG44180.14413640824337584No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41970.13692632535025995No Hit
CTCTTATTAGTTGGGATGGTGGTAGCACATCCTATGCAGACTCTGTGAAG41040.13389221806944648No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC40740.1329134737853131No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCG40370.13170635583488194No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA40110.130858110788633No Hit
GTATTATTGTGCAAAAGATAAGGGCAGTGGCTGGTACCGCTGGTTCTTCG39910.13020561459921076No Hit
TTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAGTCG38890.12687788403315725No Hit
ATAATACAAGGCGGTGTCCTCAGATCTCAGACTGTTCATTTGCAGATACA37930.12374590232393043No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA37670.1228976572776815No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC37180.12129904161359698No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37110.12107066794729919No Hit
TATTTAGCCTGGTATCAGCAAAAACCGGGGAAAGCCCCTAAGCTCCTGAT36940.12051604618629028No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT36540.11921105380744577No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG36500.1190805545695613No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC35750.11663369385922785No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAG34930.11395845948259661No Hit
GTGGTAGCACATCCTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCC34630.11297971519846324No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA33980.11085910258284092No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA33960.1107938529638987No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33830.11036973044077424No Hit
ACCATGCACTGGGTCCGTCAAGGTCCGGGGAAAGGTCTGGAGTGGGTCTC33330.1087384899672186No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT33250.1084774914914497No Hit
ATACTTACCCGCGCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACT32760.10687887582736517No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA32270.10528026016328065No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA31900.10407314221284947No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTT31490.10273552502453386No Hit
GTCTGAGATCTGAGGACACCGCCTTGTATTATTGTGCAAAAGATAAGGGC31330.10221352807299605No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC30780.10041916355208486No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)