FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3196439
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT124830.39052833481258364No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC112960.35339326043763075No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGA74510.23310315009921978No Hit
GTGTATTACTGTGCGAGAGCCTTCAATACTTATACCAGCAGCTGGTACTA70930.2219031866398827No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG65720.20560379847699267No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTACCAG63330.19812672789939054No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT61520.19246417654145753No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC55040.17219161698377475No Hit
CTACAGAAGCCTCTGAGAGGAAAGTTCTTCACCATGGACTGGACCTGGAG54660.17100279404675015No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCCGTGAAGGTTTCCTGCA52880.16543409713121385No Hit
ACAGTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA52540.16437041345071812No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA52170.16321287532782575No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC49680.15542295660890135No Hit
GGATTAATCAACCCTAATGATGGTAGCACAAACTACGCACAGAAGTTCCA49600.15517267809584354No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC48470.15163749409890193No Hit
ATTTATTACTGTCAACAAACTTACAGTACCCCGCTCACTTTCGGCGGAGG47110.1473827593769191No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG45820.1433470183538619No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT45770.14319059428320077No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT45480.1422833346733662No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA44870.1403749610113004No Hit
ACTTATACCAGCAGCTGGTACTATGACTACTGGGGCCAGGGAACCATGGT44680.13978054954278807No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT44530.1393112773308047No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT44480.13915485326014354No Hit
ACCCTAATGATGGTAGCACAAACTACGCACAGAAGTTCCAGGGCAGAGTC44480.13915485326014354No Hit
ATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTGTTGATC44430.1389984291894824No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG43750.13687106182849101No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG43280.13540067556427637No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG42510.13299174487609494No Hit
AACTTACAGTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA42500.13296046006196272No Hit
GATCTATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAGGTTCAGTG41790.1307392382585746No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41690.13042639011725235No Hit
CTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGG41450.12967555457807892No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC40410.12642193390832737No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA40380.12632807946593066No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT40190.12573366799741836No Hit
TTATTACTGTCAACAAACTTACAGTACCCCGCTCACTTTCGGCGGAGGGA39170.12254261695593127No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG38990.1219794903015512No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATTAATCAAC38970.12191692067328673No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA38530.12054038885146877No Hit
CCCCTAAGCTGTTGATCTATGCTGCATCCAGTTTGCAGAGTGGGGTCCCA37380.11694263522626272No Hit
CTTCTCTACAGAAGCCTCTGAGAGGAAAGTTCTTCACCATGGACTGGACC37360.11688006559799828No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAATTTA37250.11653593264254378No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC37200.11637950857188266No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGATTAATCAACCCTAATGATGGT36950.11559738821857697No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC36800.11512811600659359No Hit
ATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGT36680.11475269823700686No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG36400.11387672344130453No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA36120.1130007486456022No Hit
GTATTACTGTGCGAGAGCCTTCAATACTTATACCAGCAGCTGGTACTATG35940.1124376219912221No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG35790.11196834977923872No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35440.11087338128461079No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG34860.10905886206494164No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34500.10793260875618148No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC34040.10649350730609908No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC33630.10521082992667778No Hit
GGGTACTGTAAGTTTGTTGACAGTAATAAATTGCAAAATCTTCAGGTTGC33120.10361530440593422No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32830.10270804479609966No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32630.10208234851345514No Hit
GTAATAAATTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGA32540.10180078518626509No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG32330.10114380408948834No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)