FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2972778
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCGCGGCGGTCACAGACGTCAGCCTCAGGGAGA112770.37934215067522703No Hit
GTGTATTACTGTGCGAGACAACTTAAGGGGCCTCCGCTAAGGACGCGGGG105770.3557951518747784No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT81780.27509622312866955No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC80890.27210239042404105No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA66110.2223845843853796No Hit
GTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGT63320.21299942343491507No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC60820.20458978100618344No Hit
CAGTAGACCCGTCCAAGAGCCAGTTCTCCCTGAGGCTGACGTCTGTGACC59330.19957763411865936No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC58420.1965165242746011No Hit
TTATTATTGTCTCCAGTTTAATAATTACCCGCTCACTTTCGGCGGAGGGA58380.19638196999574137No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT54090.18195102358803786No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT53810.18100914363601991No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG51230.17233039264956887No Hit
GTTTAATAATTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA48810.16418985877855663No Hit
GTATTACTGTGCGAGACAACTTAAGGGGCCTCCGCTAAGGACGCGGGGGG48510.16318070168710883No Hit
CTCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG47580.16005231470362066No Hit
CTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTGAG46100.15507380638581153No Hit
TATTTGGACTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATC44150.14851428529140084No Hit
GATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCG43990.147976068175962No Hit
ATACACAGCCGTGTCCGCGGCGGTCACAGACGTCAGCCTCAGGGAGAACT43640.1467987182359396No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC43360.14585683828392165No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT41630.14003736572323933No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT41060.13811996724948852No Hit
GTCTATGGTGGGACCTTCACTGGTTACAACTGGAACTGGATCCGCCAGCC40360.13576526736944367No Hit
GTCCAAATATGGTCCCCCATGCCCATCATGCCCAGCACCTGAGTTCCTGG39720.13361239890768836No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA39570.13310782036196447No Hit
CAGTAATACACAGCCGTGTCCGCGGCGGTCACAGACGTCAGCCTCAGGGA39530.13297326608310475No Hit
GTTCAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG38670.13008034908762106No Hit
GTCTTCCTGTTCCCCCCAAAACCCAAGGACACTCTCATGATCTCCCGGAC38650.13001307194819123No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAA38210.12853297488073445No Hit
GCTGTGTATTACTGTGCGAGACAACTTAAGGGGCCTCCGCTAAGGACGCG38140.12829750489272998No Hit
CTACAATCCGTCCCTCAAGCGTCGAGTCACCATTTCAGTAGACCCGTCCA37600.12648102212812393No Hit
GTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAAAACCATCTCCAAAGC37220.12520275647895673No Hit
GTCTCCATCCTTCCTGTCTGCGTCTGCAGGAGACAGAGTCACCATCACTT37210.1251691179092418No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG36860.12399176796921937No Hit
TTATTAAACTGGAGACAATAATAAGTCGCAAAATCCTCAGGCTGCAGGCT35090.11803774112967735No Hit
TCATGAGAGTGTCCTTGGGTTTTGGGGGGAACAGGAAGACTGATGGTCCC35030.1178359097113878No Hit
GTACGTGCTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA35010.11776863257195794No Hit
ACTTAAGGGGCCTCCGCTAAGGACGCGGGGGGGCCATCTACGTGGCTATC34660.11659128263193552No Hit
CATTTACCCAGAGACAGGGAGAGGCTCTTCTGTGTGTAGTGGTTGTGCAG34650.11655764406222058No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34410.11575031838906234No Hit
CCCAAGGACACTCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGT34150.11487571557647425No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACATT34110.11474116129761457No Hit
TCCTGGAGCAGGGCGCCAGGGGGAAGACGGATGGGCCCTTGGTGGAAGCT33710.1133956185090175No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC33610.11305923281186823No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA33570.11292467853300853No Hit
CTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC33330.11211735285985029No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33240.11181460573241594No Hit
GGTCTACTGAAATGGTGACTCGACGCTTGAGGGACGGATTGTAGTTGGTG33200.11168005145355624No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA32550.10949354442208602No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG31460.10582694032315902No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC31400.10562510890486944No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA31290.10525508463800526No Hit
GTCCTTGACCAGGCAGCCCAGGGCGGCTGTGCTCTCGGAGGTGCTCCTGG30930.1040440961282679No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30790.10357315615225893No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC30590.1029003847579604No Hit
TATTGGAACCACCAACTACAATCCGTCCCTCAAGCGTCGAGTCACCATTT30280.10185758909679768No Hit
GTCCAAGAGCCAGTTCTCCCTGAGGCTGACGTCTGTGACCGCCGCGGACA30160.10145392626021855No Hit
CTTCTGTGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGA29900.10057932344763047No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)