FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4393424
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT176790.4023968549359224No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC157900.3594007771615032No Hit
GGGTATTAGCAGGTGGTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCC138840.3160177574484047No Hit
ATAATACACAGCCGTGTCCTCGGCCCTCAGGCTGTTCATTTGCAGATACA101000.22988903415650297No Hit
GTAGGGGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAG98380.22392557604274024No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG89840.20448743394673496No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT89270.20319004038763389No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC75960.17289476271809867No Hit
GTTTTCACTCTCACTATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTA73420.16711339492842028No Hit
ACATAAAGGAAGATGGAAGTGAGAAATACTATGTGGATTCTGTGAAGGGC73000.16615742072697742No Hit
GGCTAACAGTTTCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA71580.16292531747448005No Hit
CACTATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAAC69030.15712118839429112No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG67790.15429878837098354No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA66480.15131705931410216No Hit
GTGTATTATTGTGCGAGGGAATATTGGGGGACTTATCGTGACTACAATTA65930.1500651883360222No Hit
GTATTATTGTGCGAGGGAATATTGGGGGACTTATCGTGACTACAATTATT65720.14958720123530075No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG64730.14733383347475681No Hit
CAATAATACACAGCCGTGTCCTCGGCCCTCAGGCTGTTCATTTGCAGATA62490.14223530440039478No Hit
TTACTATTGTCAACAGGCTAACAGTTTCCCTCTCACTTTCGGCGGAGGGA62220.14162074955661005No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61990.14109723987486752No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC59960.1364766979012269No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC59850.13622632370561094No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG58730.13367705916842992No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC58620.1334266849728139No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA57900.1317878720560547No Hit
GTACTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAAGGGCTGGAGTGGG56840.12937517526193693No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA56840.12937517526193693No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG56680.12901099461376822No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA55370.12602926555688684No Hit
ATTGTCAACAGGCTAACAGTTTCCCTCTCACTTTCGGCGGAGGGACCAAG55000.1251870978079967No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT54940.1250505300649334No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA54650.12439045264012762No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT54550.12416283973502215No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT52440.1193602074372972No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC51710.11769863323002742No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC50990.1160598203132682No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC50700.11539974288846239No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC50020.11385197513374534No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC49370.11237249125055992No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG48200.10970942026082618No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT46880.10670492991343425No Hit
GTCTCCATCTTCTGTGTCTGCATATGTAGGGGACAGAGTCACCATCACTT46060.10483850409156957No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC45600.10379148472808451No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA45240.10297207826970492No Hit
GAGAAATACTATGTGGATTCTGTGAAGGGCCGATTCACCATCTCCAGAGA45220.10292655568868382No Hit
GCTAATACCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCCCCTACAT44190.1005821427660977No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCT43950.10003587179384461No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)