FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4453550
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT181590.4077421382941698No Hit
CTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT172330.3869497367268808No Hit
GCTTAATAGTTACCCTCCCATATTCACTTTCGGCCCTGGGACCAAAGTGG151550.3402903301860314No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC135430.30409448642094505No Hit
ATCTATATCAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGT124920.2804953351820458No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA124310.27912564134229995No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC95470.2143683129189074No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT91570.20561125394348329No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG87330.19609075905738121No Hit
GTGTATTACTGTGCGAGAGATTTCTTGGGATATTGTACTGGTGGTGTATG85390.191734683567042No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAG84390.1894892838297538No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT83600.1877154180372961No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC80040.17972179497254998No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTAATTACTACTGGAGCTGGATCCG79270.17799283717483805No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG78730.1767803213167024No Hit
CCCATATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGT78250.17570252944280404No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCG72210.16214031502958312No Hit
GATATAGATACGCCCAATCCACTCCAGTCCCTTCCCGGCGGGCTGCCGGA71480.16050117322136273No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC67100.15066632237204028No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC65890.14794938868992152No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA65170.146332700879074No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG64520.14487319104983665No Hit
TATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGAT63800.1432565032389891No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT61560.1382268078274635No Hit
ACTTATTACTGTCAACAGCTTAATAGTTACCCTCCCATATTCACTTTCGG60350.1355098741453447No Hit
ATATTGTACTGGTGGTGTATGCTCCTGGTACTTCGATCTCTGGGGCCGTG58990.13245613050263272No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTT58400.13113134465763268No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA58070.13039036274432755No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA57730.12962692683364957No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT57330.12872876693873428No Hit
GAATATGGGAGGGTAACTATTAAGCTGTTGACAGTAATAAGTTGCAAAAT55660.12497894937746293No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55630.12491158738534427No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC54670.12275600363754757No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG53700.12057796589237799No Hit
TTATTACTGTCAACAGCTTAATAGTTACCCTCCCATATTCACTTTCGGCC53270.11961244400534406No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC53010.1190286400736491No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG52400.1176589462339033No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGA52310.11745686025754734No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG52140.11707514230220835No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG51990.11673833234161513No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT51720.1161320744125473No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA51610.11588508044144559No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC51240.11505428253864895No Hit
TCATAAGGGTATCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT51100.11473992657542859No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG50500.11339268673305565No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA50490.11337023273568278No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTA48050.10789145737669949No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT47870.10748728542398761No Hit
TGTGTGAGTTGTGTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCA47720.10715047546339437No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAT47500.10665648752119096No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG47330.10627476956585197No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC46660.10477035174186886No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGG45120.10131243614644497No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC45020.10108789617271614No Hit
ATAGTTACCCTCCCATATTCACTTTCGGCCCTGGGACCAAAGTGGATATC44790.10057145423313986No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA44550.10003255829619069No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)