FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3613554
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATGAGCTGCAGAAATAGTCAGCCTCGTCCTCAGCCTGGAGCCCAGAG78630.21759741240894698No Hit
GTAATAACACCGACTGCCATTCTCATTATTACGATGTGGACGTCTGGGGC76950.21294824983935484No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG67350.18638160658454253No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT67140.1858004612633435No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT67060.1855790725695534No Hit
ATGTATTACTGTGCGACCCTGGGGCATTGTAATAACACCGACTGCCATTC65420.18104060434685632No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA64150.17752605883293845No Hit
GCCTTGGGCTGACCAAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCT63160.17478637374728592No Hit
GTGTTATTACAATGCCCCAGGGTCGCACAGTAATACATGGCGCTGTCCGG61630.1705523149785502No Hit
CTCCTGGACAGTCGGTCACCATCTCCTGCACTGGAACCAGCGATTATAAG59580.16487922970017882No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59070.16346787677726693No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA58970.1631911409100293No Hit
ATTATAAGTATGTCTCTTGGTACCAACAACACCCCGGCAAAGCCCCCAAA54860.15181729676656278No Hit
GCTCATATACGAGTAACAGCAGGGTTTTCGGCGGAGGGACCAAGGTGACC54660.15126382503208752No Hit
TCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC54300.15026757591003206No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC53230.14730650213058946No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT52920.1464486209421528No Hit
GTAATACATGGCGCTGTCCGGGGCCTTCAGACTATTCCACTGCAGGTAGG52900.14639327376870528No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGACTATTTCTGCAGCTCATAT52710.14586747562095378No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA52130.14426240759097553No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT51310.14199317347962698No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC50740.1404157790363725No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT49500.13698425428262592No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC49170.13607102592074174No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA48570.13441061071731597No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC48130.1331929729014704No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG47380.13111745389718818No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA47060.1302318991220278No Hit
GTATGTCTCTTGGTACCAACAACACCCCGGCAAAGCCCCCAAACTCATGA46510.12870985185222084No Hit
CTCCTGCACTGGAACCAGCGATTATAAGTATGTCTCTTGGTACCAACAAC46270.12804568577085051No Hit
GACCAAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCTGCTGTTACTC46110.12760290838327032No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45790.1267173536081099No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA45330.12544436861881683No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG44800.12397766852245738No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC44770.1238946477622861No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA43960.12165308723766131No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC43750.1210719419164623No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT42760.11833225683080978No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA41740.11550955098498597No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG41530.11492840566378695No Hit
TTTATGATGTCACTAATCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGC41050.11360007350104634No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT40880.11312962252674238No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG40480.11202267905779187No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG38520.10659865605993435No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA38140.10554705976443135No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT38090.10540869183081254No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC37970.1050766087901274No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT37880.10482754650961353No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA37770.10452313705565214No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA37730.10441244270875709No Hit
CATTATTACGATGTGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTC37170.10286272185222638No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36930.10219855577085606No Hit
GTATTACTGTGCGACCCTGGGGCATTGTAATAACACCGACTGCCATTCTC36760.1017281047965521No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC36660.10145136892931446No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG36450.10087022360811544No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA36430.10081487643466792No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT36220.10023373111346891No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)