FastQCFastQC Report
Sun 7 Aug 2022
EGAF00005107654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3613554
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAACACCGACTGCCATTCTCATTATTACGATGTGGACGTCTGGGGC77020.21314196494642118No Hit
TATATGAGCTGCAGAAATAGTCAGCCTCGTCCTCAGCCTGGAGCCCAGAG75200.20810537216269634No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT69010.19097542198068715No Hit
GCCTTGGGCTGACCAAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCT65700.18181546477512167No Hit
GTAATACATGGCGCTGTCCGGGGCCTTCAGACTATTCCACTGCAGGTAGG65560.181428034560989No Hit
ATGTATTACTGTGCGACCCTGGGGCATTGTAATAACACCGACTGCCATTC64110.1774153644860434No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC63890.17680654557812062No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT62300.1724064452890423No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT62090.1718252999678433No Hit
GTGTTATTACAATGCCCCAGGGTCGCACAGTAATACATGGCGCTGTCCGG61870.17121648105992052No Hit
CTCCTGGACAGTCGGTCACCATCTCCTGCACTGGAACCAGCGATTATAAG60460.16731450533186995No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC60310.1668994015310135No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC58910.1630250993896867No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG58690.16241628048176393No Hit
TCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC57180.15823756888647575No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56110.15527649510703312No Hit
ATTATAAGTATGTCTCTTGGTACCAACAACACCCCGGCAAAGCCCCCAAA52090.1441517132440805No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGACTATTTCTGCAGCTCATAT51040.14124598663808538No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA49500.13698425428262592No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA49410.13673519200211204No Hit
GCTCATATACGAGTAACAGCAGGGTTTTCGGCGGAGGGACCAAGGTGACC49230.13623706744108433No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA49200.13615404668091302No Hit
GACCAAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCTGCTGTTACTC48980.13554522777299025No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG47650.13186464073872978No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA47510.1314772105245971No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC46600.1289589141327347No Hit
CTCCTGCACTGGAACCAGCGATTATAAGTATGTCTCTTGGTACCAACAAC46050.12743686686292774No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46000.12729849892930892No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA45150.12494624405778908No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT45090.12478020253744652No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC45000.12453114025693264No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA44890.12422673080297127No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG44890.12422673080297127No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA43810.12123798343680488No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC43120.11932850595286525No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT42700.1181662153104672No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA42660.11805552096357215No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA42520.11766809074943947No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC42010.11625673782652757No Hit
GTATGTCTCTTGGTACCAACAACACCCCGGCAAAGCCCCCAAACTCATGA41300.11429191316914042No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC38810.10740119007492346No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC38330.10607285791218285No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA37990.10513195596357491No Hit
TTTATGATGTCACTAATCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGC37950.10502126161667986No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT37900.10488289368306106No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC37320.10327782565308281No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA36680.101506716102762No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT36230.10026140470019267No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG36140.10001234241967881No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)