FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5365071
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATTATAGTGGGAGTACCTACTACAACCCGTCCCAACCGTCCCTCAA401820.7489556056201306No Hit
GTAGTACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTCCAGGGAGA235130.43826074249529967No Hit
CTATTATAGTGGGAGTACCTACTACAACCCGTCCCAACCGTCCCTCAAGA205830.3836482313095204No Hit
ATAATAGATAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA186280.34720882538180764No Hit
GTCTCTGGTGGCTCCATCAGCACTAGTAGTTACTACTGGGGCTGGATCCG185950.34659373566538076No Hit
GTGTACTACTGTGCGAGACTAGAGGACCTCTGGGGGTCCTCTGACTACTG179100.3338259642789443No Hit
ACCTACTACAACCCGTCCCAACCGTCCCTCAAGAGTCGAGTCACCATGTC150490.28049954977296665No Hit
ACTACAACCCGTCCCAACCGTCCCTCAAGAGTCGAGTCACCATGTCCGTA118590.22104087718503632No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC115410.21511364900855925No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC108800.202793215597706No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT107650.20064972113136995No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA104250.19431243314394162No Hit
ATTATAGTGGGAGTACCTACTACAACCCGTCCCAACCGTCCCTCAAGAGT103950.19375326067446264No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT95430.17787276254125994No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA95180.1774067854833608No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG89620.1670434557156839No Hit
CTATAATAGATAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG87420.16294285760617147No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAATGCCAGTG87360.16283102311227568No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG85470.1593082365545582No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC82120.1530641439787097No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC81490.15188988179280385No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCACTAGTAGTTACTACTGGG76520.14262625788176894No Hit
GTACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTCCAGGGAGAACT75930.14152655202512698No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA75850.14137743936659924No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT74580.13901027591247161No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC69440.12942978760206528No Hit
GGATTGGGACTATCTATTATAGTGGGAGTACCTACTACAACCCGTCCCAA67610.12601883553824356No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA67210.12527327224560494No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA67010.12490049059928564No Hit
GTCCGTAGACACGTCCAAGAACCAGTTCTCCCTGGAGCTGAGCTCTGTGA66740.12439723537675457No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG66530.12400581464811929No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT65760.12257060530978994No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT65350.12180640293483534No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG64930.1210235614775648No Hit
GTACTTGATGAAGCTTTACAGTGATGGCGGCCACATCAAGGGGGACGGGA64590.12038983267882196No Hit
CATCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGG64080.11943923948070771No Hit
GTACTACTGTGCGAGACTAGAGGACCTCTGGGGGTCCTCTGACTACTGGG64040.11936468315144386No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT63880.11906645783438839No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC63660.11865639802343717No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA62860.1171652714381599No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG62210.11595373108762215No Hit
CAGTAGTACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTCCAGGGA61230.11412710102065751No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG60970.11364248488044242No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCAC60100.1120208847189534No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA59250.1104365627220963No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA59020.11000786382882911No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC57990.10808803835028465No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG57960.10803212110333674No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT57910.10793892569175693No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC57450.1070815279052225No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCATTGTGGTTTTCGGCGGAGG56510.10532945416752172No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGC56200.1047516426157268No Hit
GCTGTGTACTACTGTGCGAGACTAGAGGACCTCTGGGGGTCCTCTGACTA56060.10449069546330328No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC55890.10417383106393187No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA54600.1017693894451723No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG54420.10143388596348492No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)