FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2828787
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTACTGTGCGAGATTGGCTATGGTTCAACGCGACTTTGACTACTG183020.6469910954766125No Hit
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA160310.5667093351319841No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCTTTATGTCTT108750.3844403979514895No Hit
CTATTATGGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCA94250.3331816782246242No Hit
GTCTCTGGTGGCTCCATCAGCAGTACTACTTACTACTGGGGCTGGATCCG92820.3281265079343195No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCT76230.26947946239854753No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG72900.25770763228196397No Hit
ATATTACTGTGCGAGATTGGCTATGGTTCAACGCGACTTTGACTACTGGG72600.2566471070462357No Hit
ACTTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTG65780.2325378333540136No Hit
ATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGAGAACT62920.2224274927734043No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC62390.22055389819028437No Hit
GCTGTATATTACTGTGCGAGATTGGCTATGGTTCAACGCGACTTTGACTA60100.21245855555755877No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT60030.2122110996692222No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC58540.20694382433177186No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA57510.20330268768910492No Hit
ATTATGGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACC56340.19916663926976474No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG53870.19043498149560217No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA53220.18813717681819098No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT51100.18064279848571135No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG51090.1806074476445204No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC51090.1806074476445204No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC49620.17541087398945202No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG49040.1733605252003774No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT47770.16887096836912782No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC46750.16526518256765177No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC46030.162719922001904No Hit
GGGTTGGGAGTATCTATTATGGTGGGAGCACCTACTACAACCCGTCCCTC45780.16183615097213047No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGACCACTCA45060.15929089040638267No Hit
TCCGTAGACACGTCTAAGAACCAGTTCTCCCTGAAACTGAGCTCTGTGAC44030.15564975376371568No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA43900.15519019282823346No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC43650.1543064217984599No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTACTACTTACTACTGGG40910.1446202913121419No Hit
ATCTATTATGGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGT40850.14440818626499627No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA40730.14398397617070496No Hit
CAGTAATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGA40660.14373652028236839No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGACCACTC39280.13885810419801844No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC38260.1352523183965424No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACAC37980.13426249484319605No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA37410.13224749689531237No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG37320.1319293393245939No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA37280.13178793595983013No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT36280.12825285184073598No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG36270.12821750099954504No Hit
GTCTTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC36030.12736908081096243No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC35800.1265560114635708No Hit
TTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCTTTATGTCTTCG35670.12609645052808854No Hit
GTACTACTTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTG35410.12517732865712405No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA35300.1247884694040237No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT35140.12422285594496864No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC34250.12107663107897484No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT33900.11983935163729188No Hit
CCATAATAGATACTCCCAACCCACTCCAGCCCCTTCCCTGGGGGCTGGCG33250.11754154695988069No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC33220.11743549443630787No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT33160.11722338938916221No Hit
GCATGGGATGACAGCCTGAGTGGTCTTTATGTCTTCGGAACTGGGACCAA32400.11453672545865067No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA32270.1140771645231684No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC31880.11269848171672169No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC31800.11241567498719415No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG31760.1122742716224304No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT31610.11174400900456627No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31210.11032997535692861No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG30970.10948155516834601No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC30940.10937550264477319No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG30260.10697164544378916No Hit
GATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG30140.10654743534949786No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT29790.10531015590781491No Hit
TCTAAGAACCAGTTCTCCCTGAAACTGAGCTCTGTGACCGCCGCAGACAC29310.10361331553064972No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA28940.10230533440658486No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28460.10060849402941968No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)