FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2828787
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA154440.5459583913529015No Hit
GTATATTACTGTGCGAGATTGGCTATGGTTCAACGCGACTTTGACTACTG149660.5290606892636314No Hit
GTCTCTGGTGGCTCCATCAGCAGTACTACTTACTACTGGGGCTGGATCCG84400.2983610996515468No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCTTTATGTCTT82120.29030110786001206No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT81010.2863771644878176No Hit
CTATTATGGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCA80960.2862004102818629No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG79570.281286643356322No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCT67460.23847677467409176No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA64320.22737661054013614No Hit
ATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGAGAACT61890.2187863561307373No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC60690.21454425518782433No Hit
ATATTACTGTGCGAGATTGGCTATGGTTCAACGCGACTTTGACTACTGGG60120.21252925723994065No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA59330.20973654078585627No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT56530.19983830525239266No Hit
ACTTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTG56090.1982828682399912No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT51690.1827284981159769No Hit
GCTGTATATTACTGTGCGAGATTGGCTATGGTTCAACGCGACTTTGACTA51480.18198613045096715No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC50900.17993578166189253No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT49280.17420894538896No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT47540.16805789902173618No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT47320.16728018051553545No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC46950.16597219939147062No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA46790.16540658593241556No Hit
ATTATGGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACC46180.16325018461976812No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC46040.16275527284309493No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC44570.15755869918802654No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC42850.1514783545031846No Hit
TCCGTAGACACGTCTAAGAACCAGTTCTCCCTGAAACTGAGCTCTGTGAC41890.1480846737488542No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA41380.14628178084811616No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC41250.14582221991263392No Hit
CAGTAATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGA39060.13808038569181774No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC38260.1352523183965424No Hit
GATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG37340.1320000410069758No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA37050.13097486661243848No Hit
GGGTTGGGAGTATCTATTATGGTGGGAGCACCTACTACAACCCGTCCCTC36920.13051530567695624No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTACTACTTACTACTGGG36580.1293133770764642No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG35960.12712162492262585No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG35910.12694487071667113No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT35590.125813643798561No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACAC34880.12330373407400416No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC34870.12326838323281322No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC34060.12040496509634696No Hit
ATCTATTATGGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGT33940.11998075500205564No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT33710.11916768565466401No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC33430.11817786210131763No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC32790.11591540826509736No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA32770.11584470658271549No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA32610.11527909312366041No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG32550.11506698807651476No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA32390.1145013746174597No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC32300.11418321704674123No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT31510.11139050059265686No Hit
GTACTACTTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTG31140.11008251946859202No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG31050.10976436189787354No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30780.10880988918571813No Hit
CATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTG30590.10813822320309024No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG29620.1047091916075689No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA29370.10382542057779537No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA28980.10244673777134863No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG28850.10198717683586639No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)