FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3036316
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTTCCTTCACTTTCGGCCCTGGGACCAAAGTGGATA200320.6597468774659818No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT124060.40858724849455724No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC112710.3712064225199222No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA111760.368077630918521No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT101140.3331010342796995No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC85300.2809325511573894No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA81850.26957009744703775No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC76570.25218060307293444No Hit
TTATTACTGTCAGCAGTATAATAACTGGCCTTCCTTCACTTTCGGCCCTG75050.24717453651069257No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT64530.21252728635622908No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG63910.21048533815320936No Hit
GTATATGGTACTACTACTACTACTAATGTATGAAACCCACTCCAGCCCCT63590.2094314294032637No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT63210.20817991276270323No Hit
CATCTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCAGTG62750.20666491893465636No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC61050.20106602870057005No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT55870.1840058808108247No Hit
CTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT53080.17481711389723598No Hit
ATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATG49460.16289477116347573No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA48150.15858033221838572No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG46880.15439763186703886No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG46870.15436469721860308No Hit
TAGTAGTACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCT46180.15209220647653274No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTTAGCCTGGTACCAGC44990.1481729833126723No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44840.1476789635861353No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC44290.14586755792216619No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA43850.14441843339099092No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG41930.13809498089131697No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCTTCCTTCACTTTCGGCCC39980.13167272444633563No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT39660.13061881569638994No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC38520.12686426577470858No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTA37770.12439416714202342No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA37380.12310971585302716No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA37240.12264863077492594No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC37080.1221216763999531No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA36840.12133124483749386No Hit
GTGTATTACTGTGCGAGAGCCCCCCCCCCTGGTTTTGGAGTGGTTAGGGG35880.11816951858765688No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC35660.11744495632206925No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35200.11592996249402236No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC35140.11573235460340754No Hit
CAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGT34510.11365747175195204No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC34180.11257062835357057No Hit
TCTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCAGTGGC34100.11230715116608415No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG33270.10957357534591261No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT33200.109343032806862No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32970.10858553589283856No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATATCAGTGGCCCTGGT32670.1075974964397645No Hit
GTCAGAGTGTTAGCAGCAGCTTAGCCTGGTACCAGCAGAAACCTGGCCAG32530.10713641136166328No Hit
CCTCATCTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCA32310.10641184909607564No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG32250.10621424120546082No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC32210.10608250261171762No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGA32200.10604956796328181No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA32010.10542380964300158No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT31650.10423816229931271No Hit
CACCAGGGCCACTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGA31600.10407348905713372No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC31370.10331599214311027No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA31090.1023938219869078No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG30800.10143871718226957No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG30650.10094469745573255No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT30610.10081295886198932No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC30390.10008839659640169No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)